Title: Determination of tetracycline and its major degradation products by liquid chromatography with fluorescence detection
Authors: Pena, A. 
Carmona, A. 
Barbosa, A. 
Lino, C. 
Silveira, I. 
Castillo, B. 
Keywords: Tetracycline;Epitetracycline;Epianydrotetracycline;Anydrotetracycline;Liquid chromatography;Fluorescence detection
Issue Date: 1998
Citation: Journal of Pharmaceutical and Biomedical Analysis. 18:4-5 (1998) 839-845
Abstract: A liquid chromatographic method of tetracycline and its major degradation products on a C8-reversed phase column with acidic mobile phase and fluorescence detection is described. The quantification limit, measured as the amount of sample that gave a signal ten times the peak-to-peak noise of the baseline, was: 0.25 ng for tetracycline (TC) and epitetracycline (ETC), 25 ng for and 4-epianhydrotetracycline (EATC) and 50 ng for anhydrotetracycline (ATC) of injected standard. By means of this liquid chromatography (LC) assay TC, ETC, EATC and ATC as main degradation products of tetracycline, can be separated and determined with good sensitivity and specificity within 15 min.
URI: http://hdl.handle.net/10316/5822
Rights: openAccess
Appears in Collections:FFUC- Artigos em Revistas Internacionais

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