Please use this identifier to cite or link to this item:
|Title:||Targeting of sterically stabilised pH-sensitive liposomes to human T-leukaemia cells||Authors:||Fonseca, Cristina
Moreira, João N.
Ciudad, Carlos J.
Pedroso de Lima, Maria C.
|Keywords:||Nanotechnology; pH-sensitive liposomes; Targeting; Transferrin; Human T-leukaemia cells; Cancer therapy||Issue Date:||2005||Citation:||European Journal of Pharmaceutics and Biopharmaceutics. 59:2 (2005) 359-366||Abstract:||The main aim of this work was to develop novel targeted sterically stabilised pH-sensitive liposomes tailored to promote efficient intracellular delivery of therapeutic molecules into human T-leukaemia cells. Our results indicate that the targeting moiety (thiolated transferrin) was successfully coupled to the distal reactive maleimide terminus of poly(ethylene glycol)-phospholipid conjugates incorporated in the liposomal bilayer. Results from atomic force microscopy studies, performed to characterise vesicle surface topology, indicated that, to a certain extent, thiolated transferrin has the ability to associate in a non-specific manner with the lipid membrane of pegylated liposomes. This is an issue not commonly reported in the literature but which is crucial to demonstrate the targeting proof of principle. Nevertheless, fluorimetric studies together with confocal microscopy clearly demonstrate that liposomes bearing covalently coupled transferrin associate more extensively to human T-leukaemia cells in vitro than non-targeted liposomes. Cell mechanistic studies indicate that targeted liposomes bind specifically to transferrin receptors and are internalised via receptor-dependent endocytotic pathway. In addition, the biophysical features exhibited by the developed liposomes, namely their ability to promote pH-triggered cytoplasmic delivery of loaded material, make them promising delivery systems for in vivo targeting of therapeutic molecules to tumours.||URI:||http://hdl.handle.net/10316/5758||DOI:||10.1016/j.ejpb.2004.08.012||Rights:||openAccess|
|Appears in Collections:||FFUC- Artigos em Revistas Internacionais|
Show full item record
Files in This Item:
|fileca15258190a445a4a6b72e26b69a2721.pdf||446.49 kB||Adobe PDF||View/Open|
WEB OF SCIENCETM
checked on Aug 2, 2022
Page view(s) 50527
checked on Sep 14, 2022
checked on Sep 14, 2022
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.