Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/5133
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dc.contributor.authorRebelo, Isabel A.-
dc.contributor.authorPiedade, José António P.-
dc.contributor.authorBrett, Ana Maria Oliveira-
dc.date.accessioned2008-09-01T15:04:31Z-
dc.date.available2008-09-01T15:04:31Z-
dc.date.issued2004en_US
dc.identifier.citationTalanta. 63:2 (2004) 323-331en_US
dc.identifier.urihttp://hdl.handle.net/10316/5133-
dc.description.abstractA selective method based on high performance liquid chromatography with electrochemical detection (HPLC-ECD) was developed to enable simultaneous detection of 8-oxo-7,8-dihydroguanine (8-oxoGua) and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo), products of DNA oxidative damage, in the presence of uric acid (UA), a strong interferent in their electrochemical detection. The method developed consists of HPLC isocratic elution with amperometric detection on a glassy carbon electrode, enabling a detection limit for 8-oxoGua and 8-oxodGuo lower than 1 nM in standard mixtures. Detection of low concentrations up to 25 nM of 8-oxoGua and 8-oxodGuo in the presence of UA in a 104-fold higher concentration was achieved after one-step solid phase extraction (SPE). The method was tested with urine samples and it was possible to detect and quantify the presence of 8-oxoGua, and to confirm that UA was eliminated after uricase degradation and SPE. The LOD found in urine samples was about 80 nM, a value higher than in standard mixtures, due to the increase of background current in the urine matrix. The results presented here contribute to the development of a methodological approach to simultaneous determination of 8-oxoGua and 8-oxodGuo in urine samples.en_US
dc.description.urihttp://www.sciencedirect.com/science/article/B6THP-4BG45K9-1/1/203c0cf340998db82825488fecf36e47en_US
dc.format.mimetypeaplication/PDFen
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectDNA damageen_US
dc.subjectOxidative stressen_US
dc.subject8-oxoGuaen_US
dc.subject8-oxodGuoen_US
dc.subject8-oxo-7,8-Dihydroguanineen_US
dc.subject8-oxo-7,8-Dihydro-2'-deoxyguanosineen_US
dc.subjectBiomarkeren_US
dc.subjectUric aciden_US
dc.subjectHPLC-ECDen_US
dc.titleDevelopment of an HPLC method with electrochemical detection of femtomoles of 8-oxo-7,8-dihydroguanine and 8-oxo-7,8-dihydro-2'-deoxyguanosine in the presence of uric aciden_US
dc.typearticleen_US
dc.identifier.doi10.1016/j.talanta.2003.10.051-
item.grantfulltextopen-
item.fulltextCom Texto completo-
item.languageiso639-1en-
crisitem.author.deptFaculty of Sciences and Technology-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.researchunitCEMMPRE - Centre for Mechanical Engineering, Materials and Processes-
crisitem.author.orcid0000-0002-6244-0891-
Appears in Collections:FCTUC Química - Artigos em Revistas Internacionais
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