Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/5113
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dc.contributor.authorFlorescu, Monica-
dc.contributor.authorBrett, Christopher M. A.-
dc.date.accessioned2008-09-01T15:04:10Z-
dc.date.available2008-09-01T15:04:10Z-
dc.date.issued2005en_US
dc.identifier.citationTalanta. 65:2 (2005) 306-312en_US
dc.identifier.urihttp://hdl.handle.net/10316/5113-
dc.description.abstractElectrochemical glucose enzyme biosensors have been prepared on carbon film electrodes made from carbon film electrical resistors. Evaluation and characterisation of these electrodes in phosphate buffer saline solution has been carried out with and without pretreatment by cycling in perchloric acid or at fixed applied potential. Both pretreatments led to a reduction in the carbon surface oxidation peak and enabled better detection of hydrogen peroxide in the pH range of 5-7. Glucose oxidase enzyme was immobilised on the carbon surface by mixing with glutaraldehyde, bovine serum albumin and with and without Nafion. The performance of these two types of electrode was similar, that containing Nafion being more physically robust. Linear ranges were up to around 1.5 mM, with detection limits 60 [mu]M, and pretreatment of the carbon film electrode at a fixed potential of +0.9 V versus SCE for 5 min was found to be the most beneficial. Michaelis-Menten constants between 5 mM and 10 mM were found under the different experimental conditions. Coating the immobilised enzyme layer with a thin layer of Nafion was found to give similar results in the determination of glucose to mixing it but with benefits against interferences for the analysis of complex matrices, such as wine. Potentialities, for a short-term-use or disposable sensors, are indicated.en_US
dc.description.urihttp://www.sciencedirect.com/science/article/B6THP-4D268VV-4/1/7a2a399ad9f6b3fd4d087e658113f82den_US
dc.format.mimetypeaplication/PDFen
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectCarbon filmen_US
dc.subjectElectrochemical biosensoren_US
dc.subjectGlucoseen_US
dc.titleDevelopment and evaluation of electrochemical glucose enzyme biosensors based on carbon film electrodesen_US
dc.typearticleen_US
dc.identifier.doi10.1016/j.talanta.2004.07.003-
item.fulltextCom Texto completo-
item.grantfulltextopen-
item.languageiso639-1en-
crisitem.author.orcid0000-0002-1972-4434-
Appears in Collections:FCTUC Química - Artigos em Revistas Internacionais
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