Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/5100
Title: | Synthetic oligonucleotides: AFM characterisation and electroanalytical studies | Authors: | Brett, A. M. Oliveira Paquim, A. M. Chiorcea Diculescu, V. Oretskaya, T. S. |
Keywords: | DNA biosensor; Hybridisation; Adsorption; Non-specific adsorption | Issue Date: | 2005 | Citation: | Bioelectrochemistry. 67:2 (2005) 181-190 | Abstract: | One of the most important steps in designing more sensitive and stable DNA based biosensors is the immobilisation procedure of the nucleic acid probes on the transducer surface, while maintaining their conformational flexibility. MAC Mode AFM images in air demonstrated that the oligonucleotide sequences adsorb spontaneously on the electrode surface, showing the existence of pores in the adsorbed layer that reveal big parts of the electrode surface, which enables non-specific adsorption of other molecules on the uncovered areas. The electrostatic immobilisation onto a glassy carbon electrode followed by hybridisation with a complementary sequence and control with a non-complementary sequence was studied using differential pulse voltammetry and electrochemical impedance spectroscopy. Changes in the oxidation currents of guanosine and adenosine were observed after hybridisation events as well as after control experiments. Modification of the double layer capacitance that took place after hybridisation or control experiments showed that non-specific adsorption of complementary or non-complementary sequences occur allowing the formation of a mixed multilayer. | URI: | https://hdl.handle.net/10316/5100 | DOI: | 10.1016/j.bioelechem.2004.06.008 | Rights: | openAccess |
Appears in Collections: | FCTUC Química - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
file0d3b512cbf99438cbec09e9b3444e0d8.pdf | 1.39 MB | Adobe PDF | View/Open |
SCOPUSTM
Citations
15
checked on Jun 10, 2024
WEB OF SCIENCETM
Citations
14
checked on Jun 2, 2024
Page view(s) 50
412
checked on Jun 25, 2024
Download(s)
312
checked on Jun 25, 2024
Google ScholarTM
Check
Altmetric
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.