Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/5095
DC FieldValueLanguage
dc.contributor.authorLuca, S. De-
dc.contributor.authorFlorescu, M.-
dc.contributor.authorGhica, M. E.-
dc.contributor.authorLupu, A.-
dc.contributor.authorPalleschi, G.-
dc.contributor.authorBrett, C. M. A.-
dc.contributor.authorCompagnone, D.-
dc.date.accessioned2008-09-01T15:03:52Z-
dc.date.available2008-09-01T15:03:52Z-
dc.date.issued2005en_US
dc.identifier.citationTalanta. 68:2 (2005) 171-178en_US
dc.identifier.urihttps://hdl.handle.net/10316/5095-
dc.description.abstractCarbon film resistor electrodes have been evaluated as transducers for the development of multiple oxidase-based enzyme electrode biosensors. The resistor electrodes were first modified with Prussian Blue (PB) and then covered by a layer of covalently immobilized enzyme. Electrochemical impedance spectroscopy was used to characterize the interfacial behaviour of the Prussian Blue modified and enzyme electrodes; the spectra demonstrated that the access of the substrates is essentially unaltered by application of the enzyme layer. These enzyme electrodes were used to detect the substrate of the oxidase (glucose, ethanol, lactate, glutamate) via reduction of hydrogen peroxide at +50 mV versus Ag/AgCl in the low micromolar range. Response times were 1-2 min. Finally, the glucose, ethanol and lactate electrochemical biosensors were used to analyse complex food matrices--must, wine and yoghurt. Data obtained by the single standard addition method were compared with a spectrophotometric reference method and showed good correlation, indicating that the electrodes are suitable for food analysis.en_US
dc.description.urihttp://www.sciencedirect.com/science/article/B6THP-4GNTFGP-2/1/087dc22044a84cfb2a1badf317934bcaen_US
dc.format.mimetypeaplication/PDFen
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectOxidase enzymesen_US
dc.subjectCarbon film electrodesen_US
dc.subjectPrussian Blue mediatoren_US
dc.subjectFood analysisen_US
dc.titleCarbon film electrodes for oxidase-based enzyme sensors in food analysisen_US
dc.typearticleen_US
dc.identifier.doi10.1016/j.talanta.2005.06.017-
item.fulltextCom Texto completo-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextopen-
item.languageiso639-1en-
item.openairetypearticle-
item.cerifentitytypePublications-
crisitem.author.researchunitCEMMPRE - Centre for Mechanical Engineering, Materials and Processes-
crisitem.author.orcid0000-0002-1590-0816-
crisitem.author.orcid0000-0002-1972-4434-
Appears in Collections:FCTUC Química - Artigos em Revistas Internacionais
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