Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/5027
DC FieldValueLanguage
dc.contributor.authorBarsan, Madalina M.-
dc.contributor.authorBrett, Christopher M. A.-
dc.date.accessioned2008-09-01T15:02:41Z-
dc.date.available2008-09-01T15:02:41Z-
dc.date.issued2008en_US
dc.identifier.citationTalanta. 74:5 (2008) 1505-1510en_US
dc.identifier.urihttp://hdl.handle.net/10316/5027-
dc.description.abstractA new amperometric biosensor for ethanol monitoring has been developed and optimised. The biosensor uses poly(neutral red) (PNR), as redox mediator, which is electropolymerised on carbon film electrodes and alcohol oxidase (AlcOx) from Hansenula polymorpha as recognition element, immobilised by cross-linking with glutaraldehyde (GA) in the presence of bovine serum albumin (BSA) as carrier protein. Optimisation of variables affecting the system was performed and, for chronoamperometric measurements, a potential of -0.300 V versus saturated calomel electrode was chosen in 0.1 M sodium phosphate buffer saline at pH 7.5. The optimised biosensor showed a good sensitivity of 171.8 ± 14.8 nA mM-1 and the corresponding detection limit (signal-to-noise-ratio = 3) of 29.7 ± 1.5 [mu]M. Stability studies showed a good preservation of the bioanalytical properties of the sensor, 57.6% of its initial sensitivity remaining after 3 weeks (the sensor was used two to three times per week). No significant interferences were found from compounds usually present in wine. The biosensor was used for the determination of ethanol in Portuguese red and white wines.en_US
dc.description.urihttp://www.sciencedirect.com/science/article/B6THP-4PT7WW6-5/1/63b0a7a11cea967b9078c3d9acbee6e5en_US
dc.format.mimetypeaplication/PDFen
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectAlcohol oxidaseen_US
dc.subjectElectrochemical enzyme biosensoren_US
dc.subjectPoly(neutral red)en_US
dc.subjectEthanol determinationen_US
dc.titleAn alcohol oxidase biosensor using PNR redox mediator at carbon film electrodesen_US
dc.typearticleen_US
dc.identifier.doi10.1016/j.talanta.2007.09.027-
item.fulltextCom Texto completo-
item.grantfulltextopen-
item.languageiso639-1en-
crisitem.author.researchunitCEMMPRE - Centre for Mechanical Engineering, Materials and Processes-
crisitem.author.orcid0000-0002-8492-5558-
crisitem.author.orcid0000-0002-1972-4434-
Appears in Collections:FCTUC Química - Artigos em Revistas Internacionais
Files in This Item:
File Description SizeFormat
filef856b36dc8c748fe88926a0579634c4c.pdf292.55 kBAdobe PDFView/Open
Show simple item record

WEB OF SCIENCETM
Citations

69
checked on Aug 2, 2022

Page view(s)

206
checked on Oct 6, 2022

Download(s)

254
checked on Oct 6, 2022

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.