Please use this identifier to cite or link to this item:
|Title:||Forensic intoxication with clobazam: HPLC/DAD/MSD analysis||Authors:||Proença, Paula
Marques, Estela P.
Vieira, Duarte Nuno
|Keywords:||Forensic intoxication; Clobazam; HPLC/DAD/MSD||Issue Date:||2004||Citation:||Forensic Science International. 143:2-3 (2004) 205-209||Abstract:||Clobazam (Castillium®, Urbanil®), a benzodiazepine often used as an anxiolytic and in the treatment of epilepsy, is considered a relatively safe drug. The authors present a fatal case with a 49-year-old female, found dead at home. She had been undergoing psychiatric treatment and was a chronic alcoholic. The autopsy findings were unremarkable, except for multivisceral congestion, steatosis and a small piece of a plastic blister pack in the stomach. Bronchopneumonia, bronchitis and bronchiolitis were also diagnosed. Anhigh-performance liquid chromatography (HPLC)/diode array detector (DAD)/mass spectrometry detection (MSD) with electrospray method was developed in order to detect, confirm and quantify clobazam in the post-mortem samples. In the chromatographic separation, a reversed-phase column C18 (2.1×150 mm, 3.5 [mu]m) was used with a mobile phase of methanol and water, at a 0.25 ml/min flow rate. Carbonate buffer (pH 10.5) and 20 [mu]l of prazepam (100 [mu]g/ml) as internal standard were added to the samples. A simple and reliable liquid-liquid extraction method for the determination of clobazam in post-mortem samples was described. Calibration curves for clobazam were performed in blood, achieving linearity between 0.01 and 10 [mu]g/ml and a detection limit of 1.0 ng/ml. The clobazam concentration found in post-mortem blood was 3.9 [mu]g/ml, higher than the reported therapeutic concentration (0.1-0.4 [mu]g/ml). The simultaneous acquisition by photodiode array detection and mass spectrometry detection results allowed benzodiazepines to be identified with sufficient certainty.||URI:||http://hdl.handle.net/10316/4782||DOI:||10.1016/j.forsciint.2004.03.029||Rights:||openAccess|
|Appears in Collections:||FMUC Medicina - Artigos em Revistas Internacionais|
Show full item record
Files in This Item:
|file6c3cb90225ed4560be72fa83929d7740.pdf||105.69 kB||Adobe PDF||View/Open|
WEB OF SCIENCETM
checked on Aug 2, 2022
Page view(s) 20603
checked on Sep 20, 2022
checked on Sep 20, 2022
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.