Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/47554
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dc.contributor.authorPereira, Gonçalo C.-
dc.contributor.authorPereira, Susana P.-
dc.contributor.authorPereira, Claudia V.-
dc.contributor.authorLumini, José A.-
dc.contributor.authorMagalhães, José-
dc.contributor.authorAscensão, António-
dc.contributor.authorSantos, Maria S.-
dc.contributor.authorMoreno, António J.-
dc.contributor.authorOliveira, Paulo J.-
dc.date.accessioned2018-02-12T13:23:14Z-
dc.date.available2018-02-12T13:23:14Z-
dc.date.issued2012-
dc.identifier.urihttps://hdl.handle.net/10316/47554-
dc.description.abstractAlthough doxorubicin (DOX) is a very effective antineoplastic agent, its clinical use is limited by a dose-dependent, persistent and cumulative cardiotoxicity, whose mechanism remains to be elucidated. Previous works in animal models have failed to use a multi-organ approach to demonstrate that DOX-associated toxicity is selective to the cardiac tissue. In this context, the present work aims to investigate in vivo DOX cardiac, hepatic and renal toxicity in the same animal model, with special relevance on alterations of mitochondrial bioenergetics. To this end, male Wistar rats were sub-chronically (7 wks, 2 mg/Kg) or acutely (20 mg/Kg) treated with DOX and sacrificed one week or 24 hours after the last injection, respectively. Alterations of mitochondrial bioenergetics showed treatment-dependent differences between tissues. No alterations were observed for cardiac mitochondria in the acute model but decreased ADP-stimulated respiration was detected in the sub-chronic treatment. In the acute treatment model, ADP-stimulated respiration was increased in liver and decreased in kidney mitochondria. Aconitase activity, a marker of oxidative stress, was decreased in renal mitochondria in the acute and in heart in the sub-chronic model. Interestingly, alterations of cardiac mitochondrial bioenergetics co-existed with an absence of echocardiograph, histopathological or ultra-structural alterations. Besides, no plasma markers of cardiac injury were found in any of the time points studied. The results confirm that alterations of mitochondrial function, which are more evident in the heart, are an early marker of DOX-induced toxicity, existing even in the absence of cardiac functional alterations.por
dc.language.isoengpor
dc.rightsopenAccesspor
dc.subjectAnimalspor
dc.subjectAntibiotics, Antineoplasticpor
dc.subjectDoxorubicinpor
dc.subjectHeartpor
dc.subjectKidneypor
dc.subjectLiverpor
dc.subjectMalepor
dc.subjectMitochondria, Heartpor
dc.subjectMyocardiumpor
dc.subjectRatspor
dc.subjectRats, Wistarpor
dc.titleMitochondrionopathy phenotype in doxorubicin-treated Wistar rats depends on treatment protocol and is cardiac-specificpor
dc.typearticle-
degois.publication.firstPagee38867por
degois.publication.issue6por
degois.publication.titlePloS onepor
dc.peerreviewedyespor
dc.identifier.doi10.1371/journal.pone.0038867por
degois.publication.volume7por
uc.controloAutoridadeSim-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypearticle-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.fulltextCom Texto completo-
item.languageiso639-1en-
crisitem.author.deptFaculty of Sciences and Technology-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.researchunitMARE - Marine and Environmental Sciences Centre-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.orcid0000-0002-1168-2444-
crisitem.author.orcid0000-0002-6881-9392-
crisitem.author.orcid0000-0003-3575-7604-
crisitem.author.orcid0000-0002-5201-9948-
Appears in Collections:I&D CNC - Artigos em Revistas Internacionais
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