Utilize este identificador para referenciar este registo:
https://hdl.handle.net/10316/44844
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Coelho, Ana R. | - |
dc.contributor.author | Martins, Tatiana R. | - |
dc.contributor.author | Couto, Renata | - |
dc.contributor.author | Deus, Cláudia | - |
dc.contributor.author | Pereira, Cláudia V. | - |
dc.contributor.author | Simões, Rui F. | - |
dc.contributor.author | Rizvanov, Albert A. | - |
dc.contributor.author | Silva, Filomena | - |
dc.contributor.author | Cunha-Oliveira, Teresa | - |
dc.contributor.author | Oliveira, Paulo J. | - |
dc.contributor.author | Serafim, Teresa L. | - |
dc.date.accessioned | 2017-12-07T19:25:03Z | - |
dc.date.available | 2017-12-07T19:25:03Z | - |
dc.date.issued | 2017-11 | - |
dc.identifier.uri | https://hdl.handle.net/10316/44844 | - |
dc.description.abstract | Doxorubicin (DOX) is one of the most widely used anti-neoplastic agents. However, treatment with DOX is associated with cumulative cardiotoxicity inducing progressive cardiomyocyte death. Sirtuin 3 (Sirt3), a mitochondrial deacetylase, regulates the activity of proteins involved in apoptosis, autophagy and metabolism. Our hypothesis is that pharmacological modulation by berberine (BER) pre-conditioning of Sirt3 protein levels decreases DOX-induced cardiotoxicity. Our results showed that DOX induces cell death in all experimental groups. Increase in Sirt3 content by transfection-mediated overexpression decreased DOX cytotoxicity, mostly by maintaining mitochondrial network integrity and reducing oxidative stress. p53 was upregulated by DOX, and appeared to be a direct target of Sirt3, suggesting that Sirt3-mediated protection against cell death could be related to this protein. BER pre-treatment increased Sirt3 and Sirt1 protein levels in the presence of DOX and inhibited DOX-induced caspase 9 and 3-like activation. Moreover, BER modulated autophagy in DOX-treated H9c2 cardiomyoblasts. Interestingly, mitochondrial biogenesis markers were upregulated in in BER/DOX-treated cells. Sirt3 over-expression contributes to decrease DOX cytotoxicity on H9c2 cardiomyoblasts, while BER can be used as a modulator of Sirtuin function and cell quality control pathways to decrease DOX toxicity. | por |
dc.description.sponsorship | FCT | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier | por |
dc.relation | info:eu-repo/grantAgreement/FCT/COMPETE/126115/PT | por |
dc.relation | PTDC/DTP-FTO/2433/ 2014 | por |
dc.relation | POCI-01-0145-FEDER-016659 | por |
dc.relation | POCI-01- 0145-FEDER-007440 | por |
dc.relation | CENTRO- 07-ST24-FEDER-002008 | por |
dc.rights | openAccess | por |
dc.title | Berberine-induced cardioprotection and Sirt3 modulation in doxorubicin-treated H9c2 cardiomyoblasts | por |
dc.type | article | - |
degois.publication.firstPage | 2904-2923 | por |
degois.publication.lastPage | 2923 | por |
degois.publication.issue | 11 | por |
degois.publication.title | Biochimica et biophysica acta | por |
dc.relation.publisherversion | http://www.sciencedirect.com/science/article/pii/S092544391730265X | por |
dc.peerreviewed | yes | por |
dc.identifier.doi | 10.1016/j.bbadis.2017.07.030 | - |
dc.identifier.doi | 10.1016/j.bbadis.2017.07.030 | por |
degois.publication.volume | 1863 | por |
item.fulltext | Com Texto completo | - |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.openairetype | article | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0002-5982-8983 | - |
crisitem.author.orcid | 0000-0002-7382-0339 | - |
crisitem.author.orcid | 0000-0002-5201-9948 | - |
crisitem.author.orcid | 0000-0003-4924-5204 | - |
Aparece nas coleções: | I&D CNC - Artigos em Revistas Internacionais |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
29_Coelho et al 2017_final.pdf | 3.9 MB | Adobe PDF | Ver/Abrir |
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