Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/41110
DC FieldValueLanguage
dc.contributor.authorVega-Naredo, Ignacio-
dc.contributor.authorCunha-Oliveira, Teresa-
dc.contributor.authorSerafim, Teresa L.-
dc.contributor.authorSardão, Vilma A.-
dc.contributor.authorOliveira, Paulo J.-
dc.date.accessioned2017-05-03T20:10:39Z-
dc.date.available2017-05-03T20:10:39Z-
dc.date.issued2015-
dc.identifier.urihttps://hdl.handle.net/10316/41110-
dc.description.abstractMitochondria play a key role in cell death and its regulation. The permeabilization of the outer mitochondrial membrane which is mainly controlled by proteins of the BCL-2 family, is a key event that can be directly induced by p53 and results in the release of pro-apoptotic factors to the cytosol, such as cytochrome c, second mitochondria derived activator of caspases/direct inhibitor-of-apoptosis (IAP) binding protein with low pI (SMAC/Diablo), Omi serine protease (Omi/HtrA2), apoptosis inducing factor (AIF), or endonuclease G (Endo-G). Hence, the determination of subcellular localization of these proteins is extremely important to predict cell fate and elucidate the specific mechanism of apoptosis. Here we describe the procedures that can be used to study the subcellular location of different pro-apoptotic proteins to be used in basic cell biology and toxicology studies.por
dc.language.isoengpor
dc.rightsopenAccesspor
dc.subjectAnimalspor
dc.subjectApoptosis Regulatory Proteinspor
dc.subjectCell Fractionationpor
dc.subjectCell Linepor
dc.subjectCell Nucleuspor
dc.subjectCytosolpor
dc.subjectElectrophoresis, Polyacrylamide Gelpor
dc.subjectFluorescent Dyespor
dc.subjectHumanspor
dc.subjectImmunoblottingpor
dc.subjectImmunohistochemistrypor
dc.subjectImmunoprecipitationpor
dc.subjectMicepor
dc.subjectMitochondriapor
dc.subjectProtein Transportpor
dc.subjectRatspor
dc.subjectTissue Fixationpor
dc.titleAnalysis of pro-apoptotic protein trafficking to and from mitochondriapor
dc.typearticle-
degois.publication.firstPage163-80por
degois.publication.lastPage180por
degois.publication.titleMethods in molecular biology (Clifton, N.J.)por
dc.peerreviewedyespor
dc.identifier.doi10.1007/978-1-4939-1875-1_14-
dc.identifier.doi10.1007/978-1-4939-1875-1_14por
degois.publication.volume1241por
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypearticle-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.fulltextCom Texto completo-
item.languageiso639-1en-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.orcid0000-0002-7382-0339-
crisitem.author.orcid0000-0003-4924-5204-
crisitem.author.orcid0000-0001-7014-4614-
crisitem.author.orcid0000-0002-5201-9948-
Appears in Collections:I&D CNC - Artigos em Revistas Internacionais
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