Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/3842
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Gonçalves, A. M. | - |
dc.contributor.author | Schacht, E. | - |
dc.contributor.author | Matthijs, G. | - |
dc.contributor.author | Barros, M. R. Aires | - |
dc.contributor.author | Cabral, J. M. S. | - |
dc.contributor.author | Gil, M. H. | - |
dc.date.accessioned | 2008-08-29T15:27:20Z | - |
dc.date.available | 2008-08-29T15:27:20Z | - |
dc.date.issued | 1999 | en_US |
dc.identifier.citation | Enzyme and Microbial Technology. 24:1-2 (1999) 60-66 | en_US |
dc.identifier.uri | https://hdl.handle.net/10316/3842 | - |
dc.description.abstract | Recombinant cutinase from Fusarium solani pisi was covalently attached to dextran and two derivatized silica supports, Biosil-NH2 and Biosil-Dextran-NH2. Kinetic parameters were determined for all three systems as well as for soluble cutinase. Long-term stability in aqueous media was studied; dextran may have a stabilizing role not only due to the covalent links involved but also in the same way as other polyhydroxides in aqueous media. Differential scanning calorimetry analysis suggests an enhancement of conformational stability of the immobilized forms. | en_US |
dc.description.uri | http://www.sciencedirect.com/science/article/B6TG1-4165DGC-9/1/b62e6c1431e1ea508958b86755959198 | en_US |
dc.format.mimetype | aplication/PDF | en |
dc.language.iso | eng | eng |
dc.rights | openAccess | eng |
dc.subject | Conformational stability | en_US |
dc.subject | cutinase | en_US |
dc.subject | dextran | en_US |
dc.subject | immobilization | en_US |
dc.subject | thermostability | en_US |
dc.title | Stability studies of a recombinant cutinase immobilized to dextran and derivatized silica supports | en_US |
dc.type | article | en_US |
dc.identifier.doi | 10.1016/S0141-0229(98)00089-1 | - |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
Appears in Collections: | FCTUC Eng.Química - Artigos em Revistas Internacionais |
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file4798fae4af544967ac680e5c2ab60651.pdf | 234.74 kB | Adobe PDF | View/Open |
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