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Title: Ultrasonication of insulin-loaded microgel particles produced by internal gelation: impact on particle's size and insulin bioactivity
Authors: Santos, Ana C.
Cunha, Joana 
Veiga, F. 
Cordeiro-da-Silva, A. 
Ribeiro, Antonio J. 
Keywords: Hydrogels; Particle size; Physical stability; Ultrasound; Emulsion; In vitro models
Issue Date: 6-Nov-2013
Publisher: Elsevier
Citation: SANTOS, Ana C. [et. al] - Ultrasonication of insulin-loaded microgel particles produced by internal gelation: impact on particle's size and insulin bioactivity. "Carbohydrate Polymers". ISSN 0144-8617. Vol. 98 Nº. 2 (2013) p. 1397-1408
Serial title, monograph or event: Carbohydrate Polymers
Volume: 98
Issue: 2
Abstract: Alginate-dextran sulfate (ADS) microgel has been used to protect insulin from gastrointestinal attack and as a carrier to promote insulin permeation through intestinal epithelium. The throughput of ADS submicron particles generation by emulsification/internal gelation is limited by its wide size distribution. The aim of this work was to study the recovery protocol influence on ADS particles through the determination of its impact on particles’ size distribution and bioactivity. ADS particles showed a wide and multimodal distribution, characterized by a high aggregation phenomenon. In an attempt to reverse particles’ tendency to aggregate and to homogenize particle size ADS populations were submitted to ultrasonication, while particle size distribution, physical and chemical stability, and the bioactivity of entrapped insulin were investigated. After ultrasonication a narrower particle population shifted to the nanoscale, with higher physical stability and significant insulin bioactivity was obtained. Emulsification internal/gelation followed by ultrasonication constituted a valid strategy to obtain ADS particles at the submicron range, with high stability and without significantly compromising insulin bioactivity, so offering promises, under previously well established conditions, to evaluate impact of ADS particle's size on biopharmaceutical and pharmacokinetics phases.
ISSN: 0144-8617
DOI: 10.1016/j.carbpol.2013.06.063
Rights: openAccess
Appears in Collections:FFUC- Artigos em Revistas Internacionais
I&D CEFarmacêuticos - Artigos em Revistas Internacionais

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