Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/26859
DC Field | Value | Language |
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dc.contributor.author | Vicentini, Fernando Campanhã | - |
dc.contributor.author | Janegitz, Bruno C. | - |
dc.contributor.author | Brett, Christopher M. A. | - |
dc.contributor.author | Fatibello-Filho, Orlando | - |
dc.date.accessioned | 2014-09-23T13:22:59Z | - |
dc.date.available | 2014-09-23T13:22:59Z | - |
dc.date.issued | 2013-11 | - |
dc.identifier.citation | VICENTINI, Fernando Campanhã [et al.] - Tyrosinase biosensor based on a glassy carbon electrode modified with multi-walled carbon nanotubes and 1-butyl-3-methylimidazolium chloride within a dihexadecylphosphate film. "Sensors and Actuators B: Chemical". ISSN 0925-4005. Vol. 188 (2013) p. 1101-1108 | por |
dc.identifier.issn | 0925-4005 | - |
dc.identifier.uri | https://hdl.handle.net/10316/26859 | - |
dc.description.abstract | A glassy carbon electrode modified with functionalized multi-walled carbon nanotubes (MWCNT), 1-butyl-3-methylimidazolium chloride (ionic liquid = IL) and tyrosinase (Tyr) within a dihexadecylphosphate (DHP) film for the development of a biosensor is proposed. MWCNT, IL and Tyr were efficiently immobilized in the film using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) as crosslinking agents, and which was characterized by cyclic voltammetry (CV) in the presence of catechol. The IL-MWCNT nanocomposite showed good conductivity and biocompatibility with Tyr enzyme, since the biosensor presented biocatalytic activity toward the oxidation of catechol to o-quinone which was electrochemically reduced to catechol at a potential of 0.04 V. The analytical curve ranged from 4.9 × 10−6 to 1.1 × 10−3 mol L−1 with a detection limit of 5.8 × 10−7 mol L−1. The developed Tyr-IL-MWCNT-DHP/GCE biosensor showed a wide linear range, good reproducibility, sensitivity and stability and the biosensor was successfully applied to the determination of catechol in natural water samples, with satisfactory results compared with a spectrophotometric method, at the 95% confidence level. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier | por |
dc.rights | openAccess | por |
dc.subject | 1-Butyl-3-methylimidazolium chloride | por |
dc.subject | Catechol determination | por |
dc.subject | Dihexadecylphosphate | por |
dc.subject | Ionic liquid | por |
dc.subject | Multi-walled carbon nanotubes | por |
dc.subject | Tyrosinase biosensor | por |
dc.title | Tyrosinase biosensor based on a glassy carbon electrode modified with multi-walled carbon nanotubes and 1-butyl-3-methylimidazolium chloride within a dihexadecylphosphate film | por |
dc.type | article | por |
degois.publication.firstPage | 1101 | por |
degois.publication.lastPage | 1108 | por |
degois.publication.title | Sensors and Actuators B: Chemical | por |
dc.relation.publisherversion | http://www.sciencedirect.com/science/article/pii/S0925400513009143 | por |
dc.peerreviewed | Yes | por |
dc.identifier.doi | 10.1016/j.snb.2013.07.109 | - |
degois.publication.volume | 188 | por |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.author.orcid | 0000-0002-1972-4434 | - |
Appears in Collections: | FCTUC Química - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
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Tyrosinase biosensor based on a glassy carbon electrode modified.pdf | 612.26 kB | Adobe PDF | View/Open |
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