Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/20228
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dc.contributor.authorDourado, Marília-
dc.contributor.authorBento, Joana-
dc.contributor.authorMesquita, Luis-
dc.contributor.authorMarques, Alcide-
dc.contributor.authorVale-Pereira, Sofia-
dc.contributor.authorSarmento-Ribeiro, Ana Bela-
dc.contributor.authorMota-Pinto, Anabela-
dc.date.accessioned2012-07-05T17:14:54Z-
dc.date.available2012-07-05T17:14:54Z-
dc.date.issued2005-
dc.identifier.urihttp://hdl.handle.net/10316/20228-
dc.description.abstractSarcoidosis is a systemic disease of unknown aetiology, morphologically characterized by well-formed epithelioid granulomas, which show little or no central necrosis. These may be present in any organ or tissue. The lung is the most frequently and prominently involved target. The granuloma is often very sharply demarcated from the adjacent tissue and is surrounded by a mantle of lymphocytes, which mediate lysis of target cells by various mechanisms, including exocytosis of lytic proteins, perforins and granzymes. Sarcoidosis laboratorial diagnosis is usually made by SACE and Lisozyme dosages. The granzymes A and B could be two other markers of the disease, since the sarcoidosis granuloma is rich in cytotoxic and NK cells. An ELISA Kit was used to measure Granzyme A and B in serum of a normal control group (NC) (n=30), and in two groups with lung pathology: one without sarcoidosis, disease control (DC) (n=21) and other with sarcoidosis (S) (n=11). Our results showed that SACE activity is significantly augmented in S group comparing with NC and DC, respectively: 82,6+/-32,7/31,9+/-17,8 - p=0,00017 and 82,6+/-32,7/31,9+/-17,8 - p=0,00024. Lisozyme activity is significantly augmented in S and DC groups comparing with NC. Granzyme B showed a significant decrease in DC and S groups comparing with NC. Granzyme A showed a significant decrease between S/NC groups. Our results suggest that the decrease of Granzyme A and B in sarcoidotic patients could be related to an ineffective inflammatory local response related to the formation of sarcoidosis granulomas. More studies are needed, particularly in BAL.por
dc.language.isoporpor
dc.rightsopenAccesspor
dc.subjectGranzimaspor
dc.subjectSarcoidosepor
dc.titleDoseamento das granzimas A e B na sarcoidose pulmonar (estudo experimental)por
dc.title.alternativeGranzymes A and B in pulmonary sarcoidosis (experimental study)por
dc.typearticlepor
degois.publication.firstPage111por
degois.publication.lastPage133por
degois.publication.issue2por
degois.publication.titleRevista Portuguesa de Pneumologiapor
dc.peerreviewedYespor
degois.publication.volume11por
item.grantfulltextopen-
item.languageiso639-1pt-
item.fulltextCom Texto completo-
crisitem.author.deptFaculdade de Medicina, Universidade de Coimbra-
crisitem.author.researchunitCNC.IBILI-
crisitem.author.orcid0000-0002-0820-9568-
Appears in Collections:FMUC Medicina - Artigos em Revistas Nacionais
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