Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/12635
DC Field | Value | Language |
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dc.contributor.author | Silva, G. J. da | - |
dc.contributor.author | Quinteira, S. | - |
dc.contributor.author | Bértolo, E. | - |
dc.contributor.author | Sousa, J. C. | - |
dc.contributor.author | Gallego, L. | - |
dc.contributor.author | Duarte, A. | - |
dc.contributor.author | Peixe, L. | - |
dc.date.accessioned | 2010-03-01T15:12:30Z | - |
dc.date.available | 2010-03-01T15:12:30Z | - |
dc.date.issued | 2004-07 | - |
dc.identifier.citation | Journal of Antimicrobial Chemotherapy. 54:1 (2004) 255–258 | en_US |
dc.identifier.issn | 0305-7453 | - |
dc.identifier.uri | https://hdl.handle.net/10316/12635 | - |
dc.description.abstract | Objective: The main objectives of this study were to assess the clonal relatedness of Acinetobacter baumannii carbapenem-resistant isolates recovered from the Iberian Peninsula and to investigate the production of carbapenemases. Methods: One hundred and sixty-two imipenem-resistant A. baumannii isolates were collected from 1998 to 2003 in three Portuguese university hospitals. An imipenem-resistant isolate (988FFP strain) recovered in 1995 from a smaller hospital unit, was also included, as well as an OXA-40-producing A. baumannii Spanish strain (SM28). Susceptibility tests were carried out by disc diffusion and Etest methods. DNA fingerprints were obtained by PFGE of Apa I-digested chromosomal DNA. Carbapene- mase activity was determined by a bioassay and spectrophotometry. The detection of the blaOXA-40 gene was conducted through PCR analysis, cloning and nucleotide sequencing. Results: All the isolates presented a similar multi-resistance pattern, including imipenem (MIC >32 mg/L). The Iberian isolates showed an identical PFGE pattern with minor band variations, including isolate 988FFP collected in 1995. PCR results revealed a blaOXA-type gene in 65 isolates and nucleotide sequence analysis revealed the presence of the blaOXA-40 gene in seven representative Portuguese iso- lates from the various geographically dispersed hospitals. Conclusions: Our results indicate that a multi-resistant epidemic clone of A. baumannii, carrying blaOXA-40, is disseminated in the Iberian Peninsula, persisting in Portugal since 1995 | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Oxford University Press | en_US |
dc.rights | openAccess | en_US |
dc.subject | Acinetobacter spp. | en_US |
dc.subject | Epidemic clones | en_US |
dc.subject | Oxacillinases | en_US |
dc.subject | B-lactamases | en_US |
dc.title | Long-term dissemination of an OXA-40 carbapenemase-producing Acinetobacter baumannii clone in the Iberian Peninsula | en_US |
dc.type | article | en_US |
dc.identifier.doi | 10.1093/jac/dkh269 | - |
item.openairetype | article | - |
item.fulltext | Com Texto completo | - |
item.languageiso639-1 | en | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0002-7479-8540 | - |
Appears in Collections: | FFUC- Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
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Long-term dissemination of an OXA-40.pdf | 98.93 kB | Adobe PDF | View/Open |
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