Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/112575
DC Field | Value | Language |
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dc.contributor.author | Jovanović Stojanov, Sofija | - |
dc.contributor.author | Ntungwe, Epole N. | - |
dc.contributor.author | Dinić, Jelena | - |
dc.contributor.author | Podolski-Renić, Ana | - |
dc.contributor.author | Pajović, Milica | - |
dc.contributor.author | Rijo, Patrícia | - |
dc.contributor.author | Pešić, Milica | - |
dc.date.accessioned | 2024-02-01T09:44:16Z | - |
dc.date.available | 2024-02-01T09:44:16Z | - |
dc.date.issued | 2023-07-12 | - |
dc.identifier.issn | 1999-4923 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/112575 | - |
dc.description.abstract | Multidrug resistance in cancer is often mediated by P-glycoprotein. Natural compounds have been suggested as a fourth generation of P-glycoprotein inhibitors. Coleon U, isolated from Plectranthus mutabilis Codd., was reported to modulate P-glycoprotein activity but the underlying mechanism has not yet been revealed. Therefore, the effects of Coleon U on cell viability, proliferation, and cell death induction were studied in a non-small-cell lung carcinoma model comprising sensitive and multidrug-resistant cells with P-glycoprotein overexpression. P-glycoprotein activity and mitochondrial membrane potential were assessed by flow cytometry upon Coleon U, sodium-orthovanadate (an ATPase inhibitor), and verapamil (an ATPase stimulator) treatments. SwissADME was used to identify the pharmacokinetic properties of Coleon U, while P-glycoprotein expression was studied by immunofluorescence. Our results showed that Coleon U is not a P-glycoprotein substrate and is equally efficient in sensitive and multidrug-resistant cancer cells. A decrease in P-glycoprotein activity observed with Coleon U and verapamil after 72 h is antagonized in combination with sodium-orthovanadate. Coleon U induced a pronounced effect on mitochondrial membrane depolarization and showed a tendency to decrease P-glycoprotein expression. In conclusion, Coleon U-delayed effect on the decrease in P-glycoprotein activity is due to P-glycoprotein's functioning dependence on ATP production in mitochondria. | pt |
dc.language.iso | eng | pt |
dc.publisher | MDPI | pt |
dc.relation | Ministry of science, technological development and innovation of the Republic of Serbia, grant number 451-03-47/2023-01/200007 | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject | Coleon U | pt |
dc.subject | P-glycoprotein | pt |
dc.subject | cancer multidrug resistance | pt |
dc.title | Coleon U, Isolated from Plectranthus mutabilis Codd., Decreases P-Glycoprotein Activity Due to Mitochondrial Inhibition | pt |
dc.type | article | - |
degois.publication.firstPage | 1942 | pt |
degois.publication.issue | 7 | pt |
degois.publication.title | Pharmaceutics | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.3390/pharmaceutics15071942 | pt |
degois.publication.volume | 15 | pt |
dc.date.embargo | 2023-07-12 | * |
uc.date.periodoEmbargo | 0 | pt |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | open | - |
item.openairetype | article | - |
item.languageiso639-1 | en | - |
item.fulltext | Com Texto completo | - |
item.cerifentitytype | Publications | - |
Appears in Collections: | FCTUC Química - Artigos em Revistas Internacionais |
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File | Description | Size | Format | |
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Coleon-U-Isolated-from-Plectranthus-mutabilis-Codd-Decreases-PGlycoprotein-Activity-Due-to-Mitochondrial-InhibitionPharmaceutics.pdf | 7.73 MB | Adobe PDF | View/Open |
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This item is licensed under a Creative Commons License