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https://hdl.handle.net/10316/112275
Title: | Multimodal ionic liquid-based chromatographic supports for an effective RNA purification | Authors: | Carapito, Rita Bernardo, Sandra C. Pereira, Matheus M. Neves, Márcia C. Freire, Mara G. Sousa, Fani |
Keywords: | Nucleic acids; Ionic liquid; Ionic liquid-based support; Multimodal ligand; Purification | Issue Date: | 2023 | Publisher: | Elsevier | Project: | UIDB/00709/2020 UIDP/00709/2020 PTDC/BII-BBF/29496/2017 UIDB/50011/2020 UIDP/50011/2020 LA/P/0006/2020 UIDB/00102/2020 UIDP/00102/2020 |
Serial title, monograph or event: | Separation and Purification Technology | Volume: | 315 | Abstract: | Nucleic acids have been considered interesting molecules to be used as biopharmaceuticals for the treatment of various diseases, in gene therapy strategies. In particular, RNA arises as the most promising approach because it does not require access to the nucleus of cells to exert its function; however, it is quite challenging due to its labile nature. To increase the possibility of translating RNA-based technology to clinical protocols, the biomanufacturing of RNAs has been intensively exploited in the last few years. However, the standard RNA purification processes remain time-consuming and present limitations regarding recovery yield and purity. This work describes the functionalization of chromatographic silica-based supports with four ionic liquids (ILs) composed of functional moieties that can promote distinct interactions with nucleic acids. After an initial screening to evaluate the binding and elution behavior of nucleic acids in the IL-based supports, SSi[C3C3NH2Im]Cl has shown to be the most promising for further purification assays. This support was studied for the RNA purification from different samples (clarified or more complex) and has shown to be highly effective, for all the conditions studied. Generally, it is here presented a new method for RNA isolation in a single step, using an IL-based chromatographic support, able to eliminate the usage of hazardous compounds often included in standard RNA extraction protocols. | URI: | https://hdl.handle.net/10316/112275 | ISSN: | 13835866 | DOI: | 10.1016/j.seppur.2023.123676 | Rights: | openAccess |
Appears in Collections: | FCTUC Eng.Química - Artigos em Revistas Internacionais I&D CERES - Artigos em Revistas Internacionais |
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Multimodal ionic liquid-based chromatographic supports for an effective RNA purification.pdf | 4.16 MB | Adobe PDF | View/Open |
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