Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/111948
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ferreira, Isabel | - |
dc.contributor.author | Brites, Gonçalo | - |
dc.contributor.author | Silva, Ana | - |
dc.contributor.author | Caramelo, Francisco | - |
dc.contributor.author | Oliveiros, Bárbara | - |
dc.contributor.author | Neves, Bruno Miguel | - |
dc.contributor.author | Cruz, Maria Teresa | - |
dc.date.accessioned | 2024-01-17T10:00:01Z | - |
dc.date.available | 2024-01-17T10:00:01Z | - |
dc.date.issued | 2023-09 | - |
dc.identifier.issn | 0340-5761 | pt |
dc.identifier.issn | 1432-0738 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/111948 | - |
dc.description.abstract | It is well established that chemical-peptide conjugation represents the molecular initiating event (MIE) in skin sensitization. This MIE has been successfully exploited in the development of in chemico peptide reactivity assays, with the Direct Peptide Reactivity Assay (DPRA) being validated as a screening tool for skin sensitization hazard as well as an OECD test guideline. This test relies on the use of a high-performance liquid chromatography/ultraviolet detection method to quantify chemical-peptide conjugation through measurement of the depletion of two synthetic peptides containing lysine or cysteine residues, which is labor-intensive and time-consuming. To improve assay throughput, sensitivity, and accuracy, we have developed a spectrophotometric assay for skin sensitization potential based on MIE measurement-the ProtReact assay. ProtReact is also a cheaper, faster, simpler, and more accessible alternative for the DPRA, giving comparable results. A set of 106 chemicals was tested with ProtReact and the peptide depletion values compared with those reported for the DPRA. The predictive capacity of both assays was evaluated with human reference data. ProtReact and DPRA assays show similar predictive capacities for hazard identification (75% and 74%, respectively), although ProtReact showed a higher specificity (86% versus 74%, respectively) and lower sensitivity (69% versus 73%). Overall, the results show that ProtReact assay described here represents an efficient, economic, and accurate assay for the prediction of skin sensitization potential of chemical haptens. | pt |
dc.language.iso | eng | pt |
dc.publisher | Springer Nature | pt |
dc.relation | SFRH/ BD/110717/2015 | pt |
dc.relation | PD/BDE/142926/2018 | pt |
dc.relation | UIDB/04539/2020 | pt |
dc.relation | info:eu-repo/grantAgreement/UIDP/04539/2020 | pt |
dc.relation | LA/P/0058/2020 | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject | Skin sensitization | pt |
dc.subject | Direct peptide reactivity assay | pt |
dc.subject | In chemico method | pt |
dc.subject | Low-molecular-weight chemicals | pt |
dc.subject.mesh | Humans | pt |
dc.subject.mesh | Animals | pt |
dc.subject.mesh | Peptides | pt |
dc.subject.mesh | Cysteine | pt |
dc.subject.mesh | Chromatography, High Pressure Liquid | pt |
dc.subject.mesh | Animal Testing Alternatives | pt |
dc.subject.mesh | High-Throughput Screening Assays | pt |
dc.subject.mesh | Skin | pt |
dc.title | Development of an in chemico high-throughput screening method for the identification of skin sensitization potential | pt |
dc.type | article | - |
degois.publication.firstPage | 2441 | pt |
degois.publication.lastPage | 2451 | pt |
degois.publication.issue | 9 | pt |
degois.publication.title | Archives of Toxicology | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.1007/s00204-023-03550-z | pt |
degois.publication.volume | 97 | pt |
dc.date.embargo | 2023-09-01 | * |
uc.date.periodoEmbargo | 0 | pt |
item.languageiso639-1 | en | - |
item.grantfulltext | open | - |
item.fulltext | Com Texto completo | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.openairetype | article | - |
item.cerifentitytype | Publications | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | ICBR Coimbra Institute for Clinical and Biomedical Research | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.parentresearchunit | Faculty of Medicine | - |
crisitem.author.orcid | 0000-0002-2279-9461 | - |
crisitem.author.orcid | 0000-0002-4041-0376 | - |
crisitem.author.orcid | 0000-0002-0015-8604 | - |
crisitem.author.orcid | 0000-0001-7836-8161 | - |
crisitem.author.orcid | 0000-0001-9846-6754 | - |
crisitem.project.grantno | Center for Innovative Biomedicine and Biotechnology - CIBB | - |
crisitem.project.grantno | Center for Innovative Biomedicine and Biotechnology | - |
crisitem.project.grantno | Center for Innovative Biomedicine and Biotechnology - Associate Laboratory | - |
Appears in Collections: | I&D CIBB - Artigos em Revistas Internacionais FMUC Medicina - Artigos em Revistas Internacionais FFUC- Artigos em Revistas Internacionais I&D CNC - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Development-of-an-in-chemico-highthroughput-screening-method-for-the-identification-of-skin-sensitization-potentialArchives-of-Toxicology.pdf | 647.62 kB | Adobe PDF | View/Open |
Page view(s)
87
checked on Sep 11, 2024
Download(s)
27
checked on Sep 11, 2024
Google ScholarTM
Check
Altmetric
Altmetric
This item is licensed under a Creative Commons License