Title: Retrospective analysis of clinical yeast isolates in a hospital in the centre of Portugal: spectrum and revision of the identification procedures
Authors: Paulo, Cristiana 
Mourão, Cristina 
Veiga, Pedro M. 
Marques, Joana M. 
Rocha, Graça 
Alves, Ana F. 
Querol, Amparo 
Meliço-Silvestre, António A. 
Gonçalves, Isabel 
Flores, Orfeu 
Clemente, Carla 
Gonçalves, Teresa 
Keywords: Yeast infections;Molecular yeast identification;Risk factor;Candida metapsilosi;Candida orthopsilosis
Issue Date: 19-Feb-2009
Publisher: Informa Healthcare
Citation: Medical Mycology. (2009) iFirst article
Abstract: We conducted a four-year (2003-2006) retrospective study of yeasts recovered in a hospital laboratory in the centre of Portugal to evaluate the epidemiology of yeast infections. Clinical isolates and data were gathered from 751 patients corresponding to 906 episodes of yeast infection. The isolates were first identified using classical and commercial methods, routinely employed at the hospital laboratory. We then re-identified the same isolates using RFLP of the ITS 5.8S rRNA gene and sequence of the D1/D2 domain of the 26S rRNA gene. Candida parapsilosis isolates were re-identified using the Ban I digestion of the SADH gene. C. albicans was the most frequently isolated of the yeasts found in the analysed specimens, with an overall incidence of 69.6% and then in deceasing order, C. glabrata, C. tropicalis, C. parapsilosis and C. krusei. C. parapsilosis was most frequently recovered from younger patients, decreasing with age, while C. glabrata occurrence increased with age. We found an increased number of cases of fungemia per 100,000 people per year, reaching a maximum of 4.4 during 2006.
URI: http://hdl.handle.net/10316/11153
ISSN: 1369-3786
Rights: openAccess
Appears in Collections:FMUC Medicina - Artigos em Revistas Internacionais

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