Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/108375
DC Field | Value | Language |
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dc.contributor.author | Caldeira, Joana B. | - |
dc.contributor.author | Santa, Cátia | - |
dc.contributor.author | Osório, Hugo | - |
dc.contributor.author | Molinos, Maria | - |
dc.contributor.author | Manadas, Bruno | - |
dc.contributor.author | Gonçalves, Raquel | - |
dc.contributor.author | Barbosa, Mário | - |
dc.date.accessioned | 2023-08-28T08:17:49Z | - |
dc.date.available | 2023-08-28T08:17:49Z | - |
dc.date.issued | 2017-09-14 | - |
dc.identifier.uri | https://hdl.handle.net/10316/108375 | - |
dc.description.abstract | Intervertebral disc (IVD) degeneration is often the cause of low back pain. Degeneration occurs with age and is accompanied by extracellular matrix (ECM) depletion, culminating in nucleus pulpous (NP) extrusion and IVD destruction. The changes that occur in the disc with age have been under investigation. However, a thorough study of ECM profiling is needed, to better understand IVD development and age-associated degeneration. As so, iTRAQ LC-MS/MS analysis of foetus, young and old bovine NPs, was performed to define the NP matrisome. The enrichment of Collagen XII and XIV in foetus, Fibronectin and Prolargin in elder NPs and Collagen XI in young ones was independently validated. This study provides the first matrisome database of healthy discs during development and ageing, which is key to determine the pathways and processes that maintain disc homeostasis. The factors identified may help to explain age-associated IVD degeneration or constitute putative effectors for disc regeneration. | pt |
dc.language.iso | eng | pt |
dc.publisher | Springer Nature | pt |
dc.relation | Supported by: The Portuguese Foundation for Science and Technology (FCT) (Projects: PTDC/SAU-NEU/103728/2008, PTDC/NEU-NMC/0205/2012, UID/NEU/04539/2013; Post- Doc grants: SFRH/BPD/78187/2011-JC, SFRH/BD/88419/2012-CS and SFRH/BD/80577/2011-MM; and the Investigator Programme: IF/00638/2014. IPATIMUP is an Associate Laboratory of the Portuguese Ministry of Science, Technology and Higher Education and is partially supported by FCT. This work was co-financed by FEDER - Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020 - Operational Programme for Competitiveness and Internationalisation (POCI), Portugal 2020, and by Portuguese funds through FCT—Fundação para a Ciência e a Tecnologia/Ministério da Ciência, Tecnologia e Inovação in the framework of the project “Institute for Research and Innovation in Health Sciences” (POCI-01–0145- FEDER-007274). This work also had the financial support of FCT / MEC through National Funds and, when applicable, co-financed by the FEDER via the PT2020 Partnership Agreement under the 4293 Unit I&D. Last but not least, it was also financed by QREN (Quadro de Referência Estratégica Nacional) and by The National Mass Spectrometry Network (RNEM) (REDE/1506/REM/2005). | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject.mesh | Aging | pt |
dc.subject.mesh | Animals | pt |
dc.subject.mesh | Cattle | pt |
dc.subject.mesh | Chromatography, Liquid | pt |
dc.subject.mesh | Computational Biology | pt |
dc.subject.mesh | Extracellular Matrix | pt |
dc.subject.mesh | Extracellular Matrix Proteins | pt |
dc.subject.mesh | Intervertebral Disc | pt |
dc.subject.mesh | Intervertebral Disc Degeneration | pt |
dc.subject.mesh | Nucleus Pulposus | pt |
dc.subject.mesh | Reproducibility of Results | pt |
dc.subject.mesh | Tandem Mass Spectrometry | pt |
dc.subject.mesh | Proteome | pt |
dc.subject.mesh | Proteomics | pt |
dc.title | Matrisome Profiling During Intervertebral Disc Development And Ageing | pt |
dc.type | article | - |
degois.publication.firstPage | 11629 | pt |
degois.publication.issue | 1 | pt |
degois.publication.title | Scientific Reports | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.1038/s41598-017-11960-0 | pt |
degois.publication.volume | 7 | pt |
dc.date.embargo | 2017-09-14 | * |
uc.date.periodoEmbargo | 0 | pt |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | open | - |
item.fulltext | Com Texto completo | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
crisitem.author.orcid | 0000-0002-2087-4042 | - |
Appears in Collections: | I&D CNC - Artigos em Revistas Internacionais IIIUC - Artigos em Revistas Internacionais |
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File | Description | Size | Format | |
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Matrisome-Profiling-during-Intervertebral-Disc-Development-and-AgeingScientific-Reports.pdf | 7.8 MB | Adobe PDF | View/Open |
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