Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/108101
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sirak, Kendra A | - |
dc.contributor.author | Fernandes, Daniel M. | - |
dc.contributor.author | Cheronet, Olivia | - |
dc.contributor.author | Novak, Mario | - |
dc.contributor.author | Gamarra, Beatriz | - |
dc.contributor.author | Balassa, Tímea | - |
dc.contributor.author | Bernert, Zsolt | - |
dc.contributor.author | Cséki, Andrea | - |
dc.contributor.author | Dani, János | - |
dc.contributor.author | Gallina, József Zsolt | - |
dc.contributor.author | Kocsis-Buruzs, Gábor | - |
dc.contributor.author | Kővári, Ivett | - |
dc.contributor.author | László, Orsolya | - |
dc.contributor.author | Pap, Ildikó | - |
dc.contributor.author | Patay, Róbert | - |
dc.contributor.author | Petkes, Zsolt | - |
dc.contributor.author | Szenthe, Gergely | - |
dc.contributor.author | Szeniczey, Tamás | - |
dc.contributor.author | Hajdu, Tamás | - |
dc.contributor.author | Pinhasi, Ron | - |
dc.date.accessioned | 2023-08-11T09:43:40Z | - |
dc.date.available | 2023-08-11T09:43:40Z | - |
dc.date.issued | 2017-06-01 | - |
dc.identifier.issn | 0736-6205 | pt |
dc.identifier.issn | 1940-9818 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/108101 | - |
dc.description.abstract | Ancient DNA (aDNA) research involves invasive and destructive sampling procedures that are often incompatible with anthropological, anatomical, and bioarcheological analyses requiring intact skeletal remains. The osseous labyrinth inside the petrous bone has been shown to yield higher amounts of endogenous DNA than any other skeletal element; however, accessing this labyrinth in cases of a complete or reconstructed skull involves causing major structural damage to the cranial vault or base. Here, we describe a novel cranial base drilling method (CBDM) for accessing the osseous labyrinth from the cranial base that prevents damaging the surrounding cranial features, making it highly complementary to morphological analyses. We assessed this method by comparing the aDNA results from one petrous bone processed using our novel method to its pair, which was processed using established protocols for sampling disarticulated petrous bones. We show a decrease in endogenous DNA and molecular copy numbers when the drilling method is used; however, we also show that this method produces more endogenous DNA and higher copy numbers than any postcranial bone. Our results demonstrate that this minimally-invasive method reduces the loss of genetic data associated with the use of other skeletal elements and enables the combined craniometric and genetic study of individuals with archeological, cultural, and evolutionary value. | pt |
dc.language.iso | eng | pt |
dc.publisher | Eaton Publishing Co. | pt |
dc.relation | NSF grant BCS-1613577 | pt |
dc.relation | IRC Post-Graduate grant GOIPG/2013/36; | pt |
dc.relation | IRC Postdoctoral grant (GOIPD/2013/1 | pt |
dc.relation | H2020-MSCAIF- 2015 (703373) | pt |
dc.relation | János Bolyai Research Fellowship of the Hungarian Academy of Sciences (BO/00094/15/8) | pt |
dc.relation | ERC starting grant ADNABIOARC (263441) | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject | cranial base drilling method | pt |
dc.subject | petrous bone | pt |
dc.subject | ancient DNA | pt |
dc.subject | preservation of skeletal remains | pt |
dc.subject.mesh | Anthropology | pt |
dc.subject.mesh | DNA, Ancient | pt |
dc.subject.mesh | Gene Library | pt |
dc.subject.mesh | Humans | pt |
dc.subject.mesh | Minimally Invasive Surgical Procedures | pt |
dc.subject.mesh | Orthopedic Procedures | pt |
dc.subject.mesh | Petrous Bone | pt |
dc.subject.mesh | Sequence Analysis, DNA | pt |
dc.subject.mesh | Skull | pt |
dc.subject.mesh | Specimen Handling | pt |
dc.title | A minimally-invasive method for sampling human petrous bones from the cranial base for ancient DNA analysis | pt |
dc.type | article | - |
degois.publication.firstPage | 283 | pt |
degois.publication.lastPage | 289 | pt |
degois.publication.issue | 6 | pt |
degois.publication.title | BioTechniques | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.2144/000114558 | pt |
degois.publication.volume | 62 | pt |
dc.date.embargo | 2017-06-01 | * |
uc.date.periodoEmbargo | 0 | pt |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | Com Texto completo | - |
crisitem.author.researchunit | CIAS - Research Centre for Anthropology and Health | - |
crisitem.author.orcid | 0000-0002-7434-6552 | - |
Appears in Collections: | I&D CIAS - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
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A minimally-invasive method for sampling human petrous bones from the cranial base for ancient DNA analysis.pdf | 739.01 kB | Adobe PDF | View/Open |
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This item is licensed under a Creative Commons License