Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/108092
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Alabi, Olumuyiwa Samuel | - |
dc.contributor.author | Mendonça, Nuno | - |
dc.contributor.author | Adeleke, Olufemi Ezekiel | - |
dc.contributor.author | Silva, Gabriela Jorge da | - |
dc.date.accessioned | 2023-08-09T15:32:02Z | - |
dc.date.available | 2023-08-09T15:32:02Z | - |
dc.date.issued | 2017-06 | - |
dc.identifier.issn | 1680-6905 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/108092 | - |
dc.description.abstract | Background: Globally, and particularly in developing countries, the menace of anti-microbial resistance is an accelerating problem. In Nigeria, increase in bacterial resistance has been phenotypically established but due to high cost, few molecular studies have been reported. Objectives: This study screened for presence of transferable resistance genes and mobile genetic elements (MGEs) such as integron among multi-drug resistant (MDR) P. mirabilis. Methods: A total of 108 P. mirabilis strains collected from five tertiary hospitals in SouthWest Nigeria were subjected to antibiotic susceptibility study using disc-diffusion method. Transferable resistance genes and MGEs were amplified using Polymerase chain reaction (PCR) analysis and amplicons sequenced. Results: Varied resistance was observed against all the antibiotics tested. About 56% of the isolates were MDR including those from 0-12 years old children. PCR analysis revealed the presence of aac(6’)-Ib (33.3%), plasmid mediated quinolone resistance (PMQR) genes [qnrA (36.7%), acc(6’)-Ib-cr (5%)], TEM (48.3%), CTX-M (6.7%) and integrons class 1 (58.3%) and class 2 (26.7%). Sequencing analysis revealed blaTEM-1, blaCTX-M-15 associated with ISEcp1 and eight different arrays of gene cassettes: aadA1, aadA1-qacH, aadB-aadA2, aadA5, dfrA7, dfrA15, dfrA17, dfrA17-aadA5. Conclusion: Transferable resistance genes in association with MGEs are present in Nigerian P. mirabilis thus their potential in disseminating resistance. | pt |
dc.language.iso | eng | pt |
dc.publisher | Makerere University | pt |
dc.relation | Center of Pharmaceutical Studies (CEF), Faculty of Pharmacy, University of Coimbra, Portugal. | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject | Multidrug resistance | pt |
dc.subject | resistance determinants | pt |
dc.subject | integrase | pt |
dc.subject | gene cassettes | pt |
dc.subject | Proteus mirabilis | pt |
dc.subject.mesh | Adolescent | pt |
dc.subject.mesh | Adult | pt |
dc.subject.mesh | Anti-Bacterial Agents | pt |
dc.subject.mesh | Child | pt |
dc.subject.mesh | Child, Preschool | pt |
dc.subject.mesh | Disk Diffusion Antimicrobial Tests | pt |
dc.subject.mesh | Drug Resistance, Multiple, Bacterial | pt |
dc.subject.mesh | Female | pt |
dc.subject.mesh | Genes, Bacterial | pt |
dc.subject.mesh | Humans | pt |
dc.subject.mesh | Infant | pt |
dc.subject.mesh | Infant, Newborn | pt |
dc.subject.mesh | Male | pt |
dc.subject.mesh | Middle Aged | pt |
dc.subject.mesh | Nigeria | pt |
dc.subject.mesh | Proteus Infections | pt |
dc.subject.mesh | Proteus mirabilis | pt |
dc.subject.mesh | Young Adult | pt |
dc.subject.mesh | beta-Lactam Resistance | pt |
dc.title | Molecular screening of antibiotic-resistant determinants among multidrug-resistant clinical isolates of Proteus mirabilis from SouthWest Nigeria | pt |
dc.type | article | - |
degois.publication.firstPage | 356 | pt |
degois.publication.lastPage | 365 | pt |
degois.publication.issue | 2 | pt |
degois.publication.title | African Health Sciences | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.4314/ahs.v17i2.9 | pt |
degois.publication.volume | 17 | pt |
dc.date.embargo | 2017-06-01 | * |
uc.date.periodoEmbargo | 0 | pt |
item.fulltext | Com Texto completo | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.languageiso639-1 | en | - |
item.openairetype | article | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
crisitem.author.researchunit | MARE - Marine and Environmental Sciences Centre | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0002-5567-282X | - |
crisitem.author.orcid | 0000-0002-7479-8540 | - |
Appears in Collections: | I&D CNC - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Molecular-screening-of-antibioticresistant-determinants-among-multidrugresistant-clinical-isolates-of-Proteus-mirabilis-from-SouthWest-NigeriaAfrican-Health-Sciences.pdf | 492.53 kB | Adobe PDF | View/Open |
Page view(s)
75
checked on Oct 16, 2024
Download(s)
27
checked on Oct 16, 2024
Google ScholarTM
Check
Altmetric
Altmetric
This item is licensed under a Creative Commons License