Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/107380
DC Field | Value | Language |
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dc.contributor.author | Silva, Alexandre D. | - |
dc.contributor.author | Serpa, Carlos | - |
dc.contributor.author | Arnaut, Luís G. | - |
dc.date.accessioned | 2023-07-06T11:21:52Z | - |
dc.date.available | 2023-07-06T11:21:52Z | - |
dc.date.issued | 2019-02-22 | - |
dc.identifier.issn | 2045-2322 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/107380 | - |
dc.description.abstract | Photoacoustic transfection consists in the use of photoacoustic waves, generated in the thermoelastic expansion of a confined material absorbing a short pulse of a laser, to produce temporary mechanical deformations of the cell membrane and facilitate the delivery of plasmid DNA into cells. We show that high stress gradients, produced when picosecond laser pulses with a fluence of 100 mJ/cm2 are absorbed by piezophotonic materials, enable transfection of a plasmid DNA encoding Green Fluorescent Protein (gWizGFP, 3.74 MDa) in COS-7 monkey fibroblast cells with an efficiency of 5% at 20 °C, in 10 minutes. We did not observe significant cytotoxicity under these conditions. Photoacoustic transfection is scalable, affordable, enables nuclear localization and the dosage is easily controlled by the laser parameters. | pt |
dc.language.iso | eng | pt |
dc.publisher | Springer Nature | pt |
dc.relation | This work was supported by the Portuguese Science Foundation (FCT, Portugal) through projects PTDC/QEQMED/ 3521/2014 and Pest-OE/QUI/UI0313/2014. ADS thanks FCT for a PhD grant (PD/BD/128319/2017). The work has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement number 654148 Laserlab-Europe | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject.mesh | Animals | pt |
dc.subject.mesh | COS Cells | pt |
dc.subject.mesh | Cell Membrane | pt |
dc.subject.mesh | Chlorocebus aethiops | pt |
dc.subject.mesh | Green Fluorescent Proteins | pt |
dc.subject.mesh | Humans | pt |
dc.subject.mesh | Photoacoustic Techniques | pt |
dc.subject.mesh | Plasmids | pt |
dc.subject.mesh | Transfection | pt |
dc.title | Photoacoustic transfection of DNA encoding GFP | pt |
dc.type | article | - |
degois.publication.firstPage | 2553 | pt |
degois.publication.issue | 1 | pt |
degois.publication.title | Scientific Reports | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.1038/s41598-018-37759-1 | pt |
degois.publication.volume | 9 | pt |
dc.date.embargo | 2019-02-22 | * |
uc.date.periodoEmbargo | 0 | pt |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | Com Texto completo | - |
crisitem.author.researchunit | CQC - Coimbra Chemistry Centre | - |
crisitem.author.researchunit | CQC - Coimbra Chemistry Centre | - |
crisitem.author.parentresearchunit | Faculty of Sciences and Technology | - |
crisitem.author.parentresearchunit | Faculty of Sciences and Technology | - |
crisitem.author.orcid | 0000-0001-7004-0110 | - |
crisitem.author.orcid | 0000-0002-3223-4819 | - |
Appears in Collections: | I&D CQC - Artigos em Revistas Internacionais |
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File | Description | Size | Format | |
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Photoacoustic-transfection-of-DNA-encoding-GFPScientific-Reports.pdf | 2.26 MB | Adobe PDF | View/Open |
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