Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/106968
Title: Unveiling the Antifungal Potential of Two Iberian Thyme Essential Oils: Effect on C. albicans Germ Tube and Preformed Biofilms
Authors: Alves, Melissa 
Gonçalves, Maria José 
Zuzarte, Mónica 
Silva, Jorge M. Alves 
Cavaleiro, Carlos 
Cruz, Maria Teresa 
Salgueiro, Lígia 
Keywords: Thymus camphoratus; Thymus carnosus; essential oil; germ tube; biofilm; cytotoxicity
Issue Date: 2019
Publisher: Frontiers Media S.A.
Project: UID/EQU/00102/2019 
Serial title, monograph or event: Frontiers in Pharmacology
Volume: 10
Abstract: Fungal infections remain a burden worldwide, thus underpinning the need for effective new therapeutic approaches. In the present study, the antifungal effect of the essential oils of two thyme species, Thymus camphoratus and Thymus carnosus, used in traditional medicine in Portugal, as well as their major compounds was assessed. A special focus was placed on their effect on Candida albicans virulence factors. Also, the safety profile of the essential oils was assessed on keratinocytes. The essential oils were analyzed by gas chromatography (GC) and gas chromatography/mass spectroscopy (GC/MS). The minimal inhibitory and minimal fungicidal concentrations of the essential oils and their main compounds were assessed on reference and clinical strains. Also, their effect on C. albicans germ tube formation, metabolism, and biofilm disruption were considered. T. camphoratus oil was rich in 1,8-cineole and α-pinene whereas T. carnosus oil showed high amounts of borneol and camphene. Regarding the antifungal effect, both oils were more active against Cryptococcus neoformans and dermatophytes and very effective in inhibiting C. albicans germ tube formation, at doses well below their MIC and in a higher extend than the isolated compounds and fluconazole, an antifungal drug widely used in the clinic. The oils also disrupted preformed C. albicans biofilms. Furthermore, no toxicity was observed at pharmacological relevant concentrations towards keratinocytes. Our study validates the traditional uses ascribed to these Iberian species. Furthermore, it brings new insights on the antifungal potential and mechanism of action of these thyme species, thus paving the way for the development of novel effective antifungal drugs.
URI: https://hdl.handle.net/10316/106968
ISSN: 1663-9812
DOI: 10.3389/fphar.2019.00446
Rights: openAccess
Appears in Collections:FMUC Medicina - Artigos em Revistas Internacionais
I&D CIEPQPF - Artigos em Revistas Internacionais
I&D CNC - Artigos em Revistas Internacionais
I&D IBILI - Artigos em Revistas Internacionais

Show full item record

SCOPUSTM   
Citations

29
checked on Apr 1, 2024

WEB OF SCIENCETM
Citations

24
checked on Apr 2, 2024

Page view(s)

71
checked on Apr 9, 2024

Download(s)

20
checked on Apr 9, 2024

Google ScholarTM

Check

Altmetric

Altmetric


This item is licensed under a Creative Commons License Creative Commons