Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/10625
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dc.contributor.authorEskilsson, K.-
dc.contributor.authorLeal, C.-
dc.contributor.authorLindman, B.-
dc.contributor.authorMiguel, M.-
dc.contributor.authorNylander, T.-
dc.date.accessioned2009-07-10T12:54:14Z-
dc.date.available2009-07-10T12:54:14Z-
dc.date.issued2001-03-06-
dc.identifier.citationLangmuir. 17:5 (2001) 1666-1669en_US
dc.identifier.issn0743-7463-
dc.identifier.urihttp://hdl.handle.net/10316/10625-
dc.description.abstractIn this work, we report on the adsorption of complexes between DNA of different molecular weight and a cationic surfactant, cetyltrimethylammonium bromide (CTAB), on hydrophobized and hydrophilic negatively charged silica surfaces as measured by ellipsometry. We will demonstrate how the adsorption is affected by the state of the DNA−surfactant complexes formed in bulk solution. High molecular weight DNA molecules, which condense (transform from coil to globule state) on addition of small amounts of cationic surfactants, do not adsorb on hydrophilic silica prior to phase separation. However, DNA−surfactant complexes formed from low molecular weight DNA were found to adsorb. For these complexes surfactants interact with DNA, without condensation of the DNA. Adsorbed DNA−surfactant complexes can easily be removed from the hydrophilic silica surface when replacing the bulk DNA−surfactant solution with pure salt solution. At the hydrophobic surface the DNA adsorbs without addition of cationic surfactant. However, with addition of a very low amount of surfactant, a rapid increase in adsorbed amount and a simultaneous decrease in adsorbed layer thickness are observed. This compaction of the adsorbed layer is to some extent reversible when replacing the bulk DNA−surfactant solution with pure salt solution.en_US
dc.language.isoengen_US
dc.publisherAmerican Chemical Societyen_US
dc.rightsopenAccesseng
dc.titleDNA−Surfactant Complexes at Solid Surfacesen_US
dc.typearticleen_US
dc.identifier.doi10.1021/la000993e-
uc.controloAutoridadeSim-
item.grantfulltextopen-
item.languageiso639-1en-
item.fulltextCom Texto completo-
crisitem.author.deptFaculty of Sciences and Technology-
crisitem.author.deptFaculty of Sciences and Technology-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.researchunitCQC - Coimbra Chemistry Centre-
crisitem.author.researchunitCQC - Coimbra Chemistry Centre-
crisitem.author.parentresearchunitFaculty of Sciences and Technology-
crisitem.author.parentresearchunitFaculty of Sciences and Technology-
crisitem.author.orcid0000-0002-7493-9112-
crisitem.author.orcid0000-0002-1601-5501-
Appears in Collections:FCTUC Química - Artigos em Revistas Internacionais
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