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Title: Evaluation of dentinogenesis inducer biomaterials: an in vivo study
Authors: Paula, Anabela B. 
Laranjo, Mafalda 
Marto, Carlos Miguel 
Paulo, Siri Folques Vicente de 
Abrantes, Ana M. 
Fernandes, Bruno
Casalta-Lopes, João 
Ferreira, Manuel Marques 
Botelho, Maria Filomena 
Carrilho, Eunice 
Keywords: Biomaterials; Dentinogenesis; Dental pulp; Odontoblast; Pulp capping
Issue Date: 2020
Publisher: Faculdade de Odontologia de Bauru da Universidade de Sao Paulo
Project: GAI 2013 (Faculty of Medicine of the University of Coimbra) 
PEst-C/SAU/ UI3282/2013 
Serial title, monograph or event: Journal of Applied Oral Science
Volume: 28
Abstract: When exposure of the pulp to external environment occurs, reparative dentinogenesis can be induced by direct pulp capping to maintain pulp tissue vitality and function. These clinical situations require the use of materials that induce dentin repair and, subsequently, formation of a mineralized tissue. Objective: This work aims to assess the effect of tricalcium silicate cements and mineral trioxide aggregate cements, including repairing dentin formation and inflammatory reactions over time after pulp exposure in Wistar rats. Methodology: These two biomaterials were compared with positive control groups (open cavity with pulp tissue exposure) and negative control groups (no intervention). The evaluations were performed in three stages; three, seven and twenty-one days, and consisted of an imaging (nuclear medicine) and histological evaluation (H&E staining, immunohistochemistry and Alizarin Red S). Results: The therapeutic effect of these biomaterials was confirmed. Nuclear medicine evaluation demonstrated that the uptake of 99mTc- Hydroxymethylene diphosphonate (HMDP) showed no significant differences between the different experimental groups and the control, revealing the nonoccurrence of differences in the phosphocalcium metabolism. The histological study demonstrated that in mineral trioxide aggregate therapies, the presence of moderate inflammatory infiltration was found after three days, decreasing during follow-ups. The formation of mineralized tissue was only verified at 21 days of follow-up. The tricalcium silicate therapies demonstrated the presence of a slight inflammatory infiltration on the third day, increasing throughout the follow-up. The formation of mineralized tissue was observed in the seventh follow-up day, increasing over time. Conclusions: The mineral trioxide aggregate (WhiteProRoot®MTA) and tricalcium silicate (Biodentine™) present slight and reversible inflammatory signs in the pulp tissue, with the formation of mineralized tissue. However, the exacerbated induction of mineralized tissue formation with the tricalcium silicate biomaterial may lead to the formation of pulp calcifications
ISSN: 1678-7765
DOI: 10.1590/1678-7757-2019-0023
Rights: openAccess
Appears in Collections:FMUC Medicina - Artigos em Revistas Internacionais
I&D CIBIT - Artigos em Revistas Internacionais

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