Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/106214
DC FieldValueLanguage
dc.contributor.authorGomes, Laidson P.-
dc.contributor.authorAnjo, Sandra I.-
dc.contributor.authorManadas, Bruno-
dc.contributor.authorCoelho, Ana V.-
dc.contributor.authorPaschoalin, Vania M F-
dc.date.accessioned2023-03-27T08:06:21Z-
dc.date.available2023-03-27T08:06:21Z-
dc.date.issued2019-12-28-
dc.identifier.issn1422-0067pt
dc.identifier.urihttps://hdl.handle.net/10316/106214-
dc.description.abstractThe well-known antimicrobial effects of chitosan (CS) polymers make them a promising adjuvant in enhancing antibiotic effectiveness against human pathogens. However, molecular CS antimicrobial mechanisms remain unclear, despite the insights presented in the literature. Thus, the aim of the present study was to depict the molecular effects implicated in the interaction of low or medium molecular mass CS polymers and their nanoparticle-counterparts against Escherichia coli. The differential E. coli proteomes sensitized to either CS polymers or nanoparticles were investigated by nano liquid chromatography-mass spectrometry (micro-LC-MS/MS). A total of 127 proteins differentially expressed in CS-sensitized bacteria were predominantly involved in (i) structural functions associated to the stability of outer membrane, (ii) increment of protein biosynthesis due to high abundance of ribosomal proteins and (iii) activation of biosynthesis of amino acid and purine metabolism pathways. Antibacterial activity of CS polymers/nanoparticles seems to be triggered by the outer bacterial membrane disassembly, leading to increased protein biosynthesis by diverting the metabolic flux to amino acid and purine nucleotides supply. Understanding CS-antibacterial molecular effects can be valuable to optimize the use of CS-based nanomaterials in food decontamination, and may represent a breakthrough on CS nanocapsules-drug delivery devices for novel antibiotics, as the chitosan-disassembly of bacteria cell membranes can potentialize antibiotic effects.pt
dc.description.sponsorshipThis research was funded by Fundação Carlos Chagas Filho de Apoio à Pesquisa do Estado do Rio de Janeiro—FAPERJ Grants: Pos-doc Nota 10 program, PDR-10-E-26/202.319/2017; CNE, E-26/203.039/2015; CNE, E-26/202.815/2018; Sediadas E-26/010002864/2014 and Nottinghan/Birminghan, E-26/010.002673/2014. This study was partially supported by: Project LISBOA-01-0145-FEDER-007660 (Microbiologia Molecular, Estrutural e Celular) funded by FEDER funds through COMPETE2020—Programa Operacional Competitividade e Internacionalização (POCI), by ONEIDA project (LISBOA-01-0145-FEDER-016417) co-funded by FEEI—“Fundos Europeus Estruturais e de Investimento” from “Programa Operacional Regional Lisboa 2020” and by national funds through “FCT—Fundação para a Ciência e a Tecnologia”. This work was partially supported by: by the European Regional Development Fund (ERDF) through the COMPETE 2020—Operational Programme for Competitiveness and Internationalisation and Portuguese national funds via FCT – Fundação para a Ciência e a Tecnologia, I.P., OE FCT/MCTES (PIDDAC) under projects: POCI-01-0145-FEDER-007440 (strategic project UID/NEU/04539/2019) and POCI-01-0145-FEDER-029311 (ref.: PTDC/BTM-TEC/29311/2017).-
dc.language.isoengpt
dc.publisherMDPIpt
dc.rightsopenAccesspt
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt
dc.subjectantibiotic e ectpt
dc.subjectanti-microbial molecular mechanismpt
dc.subjectchitosan polymerpt
dc.subjectgene ontologypt
dc.subjectKEGGpt
dc.subjectmetaboanalystpt
dc.subjectmicro-LC-MS/MSpt
dc.subjectSTRING analysispt
dc.subjectultrasonicated-cs nanoparticlespt
dc.subject.meshAnti-Bacterial Agentspt
dc.subject.meshBacterial Outer Membrane Proteinspt
dc.subject.meshChitosanpt
dc.subject.meshEscherichia colipt
dc.subject.meshNanoparticlespt
dc.subject.meshProteomept
dc.titleProteomic Analyses Reveal New Insights on the Antimicrobial Mechanisms of Chitosan Biopolymers and Their Nanosized Particles against Escherichia colipt
dc.typearticle-
degois.publication.firstPage225pt
degois.publication.issue1pt
degois.publication.titleInternational Journal of Molecular Sciencespt
dc.peerreviewedyespt
dc.identifier.doi10.3390/ijms21010225pt
degois.publication.volume21pt
dc.date.embargo2019-12-28*
uc.date.periodoEmbargo0pt
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypearticle-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.fulltextCom Texto completo-
item.languageiso639-1en-
crisitem.author.orcid0000-0002-2087-4042-
Appears in Collections:I&D CNC - Artigos em Revistas Internacionais
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