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https://hdl.handle.net/10316/106193
Title: | (+)-Limonene 1,2-Epoxide-Loaded SLNs: Evaluation of Drug Release, Antioxidant Activity, and Cytotoxicity in an HaCaT Cell Line | Authors: | Souto, Eliana B. Zielińska, Aleksandra Souto, Selma B Durazzo, Alessandra Lucarini, Massimo Santini, Antonello Atanasov, Atanas G. Marques, Conrado Andrade, Luciana N. Severino, Patrícia |
Keywords: | (+)-limonene 1; 2-epoxide; monoterpene; Imwitor 900K-SLN; lipid peroxidation; cytotoxicity; HaCaT cell line | Issue Date: | 20-Feb-2020 | Publisher: | MDPI | Project: | UIDB/04469/2020 | Serial title, monograph or event: | International Journal of Molecular Sciences | Volume: | 21 | Issue: | 4 | Abstract: | In this work, we developed a solid lipid nanoparticle (SLN) formulation with (+)-limonene 1,2-epoxide and glycerol monostearate (Lim-SLNs), stabilized with Poloxamer® 188 in aqueous dispersion to modify the release profile of the loaded monoterpene derivative. We also evaluated the role of SLNs in lipid peroxidation and cytotoxicity in a spontaneously transformed aneuploid immortal keratinocyte cell line from adult human skin (the HaCaT cell line). For the cell viability assay, the colorimetric 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was used. Lim-SLNs with a loading capacity and encapsulation efficiency of 0.39% and 63%, respectively, were produced by high pressure homogenization. A mean particle size of 194 ± 3.4 nm and polydispersity index of 0.244 were recorded for the loaded Lim-SLNs, as compared to 203 ± 1.5 nm (PI 0.213) for the non-loaded (blank) SLNs. The loading of the monoterpene derivative into glycerol monostearate SLNs fitted into the zero-order kinetics, and ameliorated both lipid peroxidation and cytotoxicity in a keratinocyte cell line. A promising formulation for antioxidant and anti-tumoral activities is here proposed. | URI: | https://hdl.handle.net/10316/106193 | ISSN: | 1422-0067 | DOI: | 10.3390/ijms21041449 | Rights: | openAccess |
Appears in Collections: | FFUC- Artigos em Revistas Internacionais |
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limonene-12epoxideloaded-slns-Evaluation-of-drug-release-antioxidant-activity-and-cytotoxicity-in-an-HaCaT-cell-lineInternational-Journal-of-Molecular-Sciences.pdf | 1.19 MB | Adobe PDF | View/Open |
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