Utilize este identificador para referenciar este registo:
https://hdl.handle.net/10316/105848
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Pádua, Diana | - |
dc.contributor.author | Barros, Rita | - |
dc.contributor.author | Amaral, Ana Luísa | - |
dc.contributor.author | Mesquita, Patrícia | - |
dc.contributor.author | Freire, Ana Filipa | - |
dc.contributor.author | Sousa, Mafalda | - |
dc.contributor.author | Maia, André Filipe | - |
dc.contributor.author | Caiado, Inês | - |
dc.contributor.author | Fernandes, Hugo | - |
dc.contributor.author | Pombinho, António | - |
dc.contributor.author | Pereira, Carlos Filipe | - |
dc.contributor.author | Almeida, Raquel | - |
dc.date.accessioned | 2023-03-10T13:06:34Z | - |
dc.date.available | 2023-03-10T13:06:34Z | - |
dc.date.issued | 2020-02-20 | - |
dc.identifier.issn | 2072-6694 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/105848 | - |
dc.description.abstract | Gastric cancer remains a serious health burden with few therapeutic options. Therefore, the recognition of cancer stem cells (CSCs) as seeds of the tumorigenic process makes them a prime therapeutic target. Knowing that the transcription factors SOX2 and OCT4 promote stemness, our approach was to isolate stem-like cells in human gastric cancer cell lines using a traceable reporter system based on SOX2/OCT4 activity (SORE6-GFP). Cells transduced with the SORE6-GFP reporter system were sorted into SORE6+ and SORE6- cell populations, and their biological behavior characterized. SORE6+ cells were enriched for SOX2 and exhibited CSC features, including a greater ability to proliferate and form gastrospheres in non-adherent conditions, a larger in vivo tumor initiating capability, and increased resistance to 5-fluorouracil (5-FU) treatment. The overexpression and knockdown of SOX2 revealed a crucial role of SOX2 in cell proliferation and drug resistance. By combining the reporter system with a high-throughput screening of pharmacologically active small molecules we identified monensin, an ionophore antibiotic, displaying selective toxicity to SORE6+ cells. The ability of SORE6-GFP reporter system to recognize cancer stem-like cells facilitates our understanding of gastric CSC biology and serves as a platform for the identification of powerful therapeutics for targeting gastric CSCs. | pt |
dc.language.iso | eng | pt |
dc.publisher | MDPI | pt |
dc.relation | project “Institute for Research and Innovation in Health Sciences” (POCI-01-0145-FEDER-007274) | pt |
dc.relation | project NORTE-07-0124-FEDER-000029 supported by Norte Portugal Regional Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF) | pt |
dc.relation | POCI-01-0145-FEDER-029017 | pt |
dc.relation | POCI-01-0145-FEDER-016390 | pt |
dc.relation | PhD fellowship (SFRH/BD/146186/2019) | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject | cancer stem cells | pt |
dc.subject | gastric cancer | pt |
dc.subject | SOX2 | pt |
dc.subject | monensin | pt |
dc.subject | SORE6-GFP reporter system | pt |
dc.subject | drug resistance | pt |
dc.subject | high-throughput screening | pt |
dc.title | A SOX2 Reporter System Identifies Gastric Cancer Stem-Like Cells Sensitive to Monensin | pt |
dc.type | article | - |
degois.publication.firstPage | 495 | pt |
degois.publication.issue | 2 | pt |
degois.publication.title | Cancers | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.3390/cancers12020495 | pt |
degois.publication.volume | 12 | pt |
dc.date.embargo | 2020-02-20 | * |
uc.date.periodoEmbargo | 0 | pt |
item.openairetype | article | - |
item.fulltext | Com Texto completo | - |
item.languageiso639-1 | en | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0002-9724-1382 | - |
Aparece nas coleções: | I&D CNC - Artigos em Revistas Internacionais |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
A-SOX2-reporter-system-identifies-gastric-cancer-stemlike-cells-sensitive-to-monensinCancers.pdf | 3.76 MB | Adobe PDF | Ver/Abrir |
Citações SCOPUSTM
32
Visto em 15/jul/2024
Citações WEB OF SCIENCETM
28
Visto em 2/jul/2024
Visualizações de página
90
Visto em 16/jul/2024
Downloads
28
Visto em 16/jul/2024
Google ScholarTM
Verificar
Altmetric
Altmetric
Este registo está protegido por Licença Creative Commons