Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/105816
Title: Choroidal and retinal structural, cellular and vascular changes in a rat model of Type 2 diabetes
Authors: Campos, António 
Martins, João 
Campos, Elisa J. 
Silva, Rufino 
Ambrósio, António Francisco 
Keywords: Type 2 diabetes; Choroid Retina; Choroidal thickness; Microglia; VEGFR2
Issue Date: Dec-2020
Publisher: Elsevier
Project: info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID/NEU/04539/2013/PT 
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID/NEU/04539/2019/PT 
UIDB/04539/2020 
UIDP/04539/2020 
CENTRO-01-0145-FEDER-000008: BrainHealth 2020 
metadata.degois.publication.title: Biomedicine and Pharmacotherapy
metadata.degois.publication.volume: 132
Abstract: Increasing evidence points to inflammation as a key factor in the pathogenesis of diabetic retinopathy (DR). Choroidal changes in diabetes have been reported and several attempts were made to validate in vivo choroidal thickness (CT) as a marker of retinopathy. We aimed to study choroidal and retinal changes associated with retinopathy in an animal model of spontaneous Type 2 diabetes, Goto-Kakizaki (GK) rats. Sclerochoroidal whole mounts and cryosections were prepared from 52-week-old GK and age-matched control Wistar Han rats. CT was measured by optical coherence tomography. Microglia reactivity, pericyte and endothelial cells distribution, and immunoreactivity of vascular endothelial growth factor (VEGF) and VEGF receptor 2 (VEGFR2) were evaluated by immunofluorescence. Choroidal vessels were visualized by direct perfusion with 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (Dil). Choroidal vascular density was evaluated by fluorescence microscopy. GK rats had increased CT (58.40 ± 1.15 μm versus 50.90 ± 1.58 μm, p < 0.001), reduced vascular density of the choriocapillaris (CC) (p = 0.045), increased Iba1+ cells density in the outer retina (p = 0.003) and increased VEGFR2 immunoreactivity in most retinal layers (p = 0.021 to 0.037). Choroidal microglial cells and pericytes showed polarity in their distribution, sparing the innermost choroid. This cell-free gap in the inner choroid was more pronounced in GK rats. In summary, GK rats have increased CT with decreased vascular density in the innermost choroid, increased VEGFR2 immunoreactivity in the retina and increased Iba1+ cells density in the outer retina.
URI: https://hdl.handle.net/10316/105816
ISSN: 07533322
DOI: 10.1016/j.biopha.2020.110811
Rights: openAccess
Appears in Collections:I&D ICBR - Artigos em Revistas Internacionais
I&D CIBB - Artigos em Revistas Internacionais
I&D CIBIT - Artigos em Revistas Internacionais
I&D ICNAS - Artigos em Revistas Internacionais

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