Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/105528
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Silva, Amélia M. | - |
dc.contributor.author | Martins-Gomes, Carlos | - |
dc.contributor.author | Souto, Eliana B. | - |
dc.contributor.author | Schäfer, Judith | - |
dc.contributor.author | Santos, João A. | - |
dc.contributor.author | Bunzel, Mirko | - |
dc.contributor.author | Nunes, Fernando M. | - |
dc.date.accessioned | 2023-03-06T10:32:32Z | - |
dc.date.available | 2023-03-06T10:32:32Z | - |
dc.date.issued | 2020-06-03 | - |
dc.identifier.issn | 2076-3921 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/105528 | - |
dc.description.abstract | Thymus zygis subsp. zygis is an endemic Portuguese plant belonging to the Thymus zygis species. Although T. zygis is commonly used as a condiment and as a medicinal herb, a detailed description of the polyphenol composition of hydroethanolic (HE) and aqueous decoction (AD) extracts is not available. In this work, we describe for the first time a detailed phenolic composition of Thymus zygis subsp. zygis HE and AD extracts, together with their antioxidant, anti-proliferative and anti-inflammatory activities. Unlike other Thymus species, T. zygis subsp. zygis extracts contain higher amounts of luteolin-(?)-O-hexoside. However, the major phenolic compound is rosmarinic acid, and high amounts of salvianolic acids K and I were also detected. T. zygis subsp. zygis extracts exhibited significant scavenging activity of ABTS+, hydroxyl (•OH), and nitric oxide (NO) radicals. Regarding the anti-proliferative/cytotoxic effect, tested against Caco-2 and HepG2 cells, the AD extract only slightly reduced cell viability at higher concentrations (IC50 > 600 µg/mL, 48 h exposure), denoting very low toxicity, while the HE extract showed a high anti-proliferative effect, especially at 48 h exposure (IC50 of 85.01 ± 15.10 μg/mL and 82.19 ± 2.46 μg/mL, for Caco-2 and HepG2, respectively). At non-cytotoxic concentrations, both extracts reduced the nitric oxide (NO) release by lipopolysaccharide (LPS)-stimulated RAW 264.7 cells (at 50 μg/mL, HE and AD extracts inhibited NO release in ~89% and 48%, respectively). In conclusion, the results highlight the non-toxic effect of aqueous extracts, both resembling the consumption of antioxidants in foodstuff or in functional food. Furthermore, the HE extract of T. zygis subsp. zygis is a source of promising molecules with antioxidant, anti-inflammatory and anticancer activities, highlighting its potential as a source of bioactive ingredients for nutraceutical and pharmaceutical industries. | pt |
dc.description.sponsorship | INTERACT project–“Integrative Research in Environment, Agro-Chains and Technology”, no. NORTE-01-0145- FEDER-000017, in its line of research entitled ISAC, co-financed by the European Regional Development Fund (ERDF) through NORTE 2020 (North Regional Operational Program 2014/2020 | pt |
dc.language.iso | eng | pt |
dc.publisher | MDPI | pt |
dc.relation | SFRH/BD/145855/2019 | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB/04033/2020 | pt |
dc.relation | info:eu-repo/grantAgreement/UIDB/00616/2020 | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB/04469/2020 | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject | Thymus zygis subsp. zygis | pt |
dc.subject | phenolic profiling | pt |
dc.subject | aqueous decoction | pt |
dc.subject | hydroethanolic extract | pt |
dc.subject | luteolin-O-hexoside | pt |
dc.subject | anti-proliferative activity | pt |
dc.subject | radical scavenging activity | pt |
dc.subject | antioxidant | pt |
dc.subject | anti-inflammatory activity | pt |
dc.title | Thymus zygis subsp. zygis an Endemic Portuguese Plant: Phytochemical Profiling, Antioxidant, Anti-Proliferative and Anti-Inflammatory Activities | pt |
dc.type | article | - |
degois.publication.firstPage | 482 | pt |
degois.publication.issue | 6 | pt |
degois.publication.title | Antioxidants | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.3390/antiox9060482 | pt |
degois.publication.volume | 9 | pt |
dc.date.embargo | 2020-06-03 | * |
uc.date.periodoEmbargo | 0 | pt |
item.fulltext | Com Texto completo | - |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.openairetype | article | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
crisitem.author.researchunit | CEGOT – Centre of Studies on Geography and Spatial Planning | - |
crisitem.author.orcid | 0000-0002-9737-6017 | - |
crisitem.author.orcid | 0000-0003-0922-1216 | - |
crisitem.project.grantno | Centre for the Research and Technology of Agro-Environmental and Biological Sciences | - |
crisitem.project.grantno | Chemistry Center - Vila Real | - |
crisitem.project.grantno | Centre of Biological Engineering of the University of Minho | - |
Appears in Collections: | FFUC- Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Thymus-zygis-subsp-zygis-an-endemic-portuguese-plant-Phytochemical-profiling-antioxidant-antiproliferative-and-antiinflammatory-activitiesAntioxidants.pdf | 1.89 MB | Adobe PDF | View/Open |
SCOPUSTM
Citations
41
checked on Jul 15, 2024
WEB OF SCIENCETM
Citations
38
checked on Jul 2, 2024
Page view(s)
78
checked on Jul 24, 2024
Download(s)
34
checked on Jul 24, 2024
Google ScholarTM
Check
Altmetric
Altmetric
This item is licensed under a Creative Commons License