Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/104862
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dc.contributor.authorBrignole, Chiara-
dc.contributor.authorBensa, Veronica-
dc.contributor.authorFonseca, Nuno A.-
dc.contributor.authorDel Zotto, Genny-
dc.contributor.authorBruno, Silvia-
dc.contributor.authorCruz, Ana F.-
dc.contributor.authorMalaguti, Fabiana-
dc.contributor.authorCarlini, Barbara-
dc.contributor.authorMorandi, Fabio-
dc.contributor.authorCalarco, Enzo-
dc.contributor.authorPerri, Patrizia-
dc.contributor.authorMoura, Vera-
dc.contributor.authorEmionite, Laura-
dc.contributor.authorCilli, Michele-
dc.contributor.authorDe Leonardis, Francesco-
dc.contributor.authorTondo, Annalisa-
dc.contributor.authorAmoroso, Loredana-
dc.contributor.authorConte, Massimo-
dc.contributor.authorGaraventa, Alberto-
dc.contributor.authorSementa, Angela R.-
dc.contributor.authorCorrias, Maria V.-
dc.contributor.authorPonzoni, Mirco-
dc.contributor.authorMoreira, João N.-
dc.contributor.authorPastorino, Fabio-
dc.date.accessioned2023-01-26T11:19:52Z-
dc.date.available2023-01-26T11:19:52Z-
dc.date.issued2021-06-02-
dc.identifier.issn1756-9966pt
dc.identifier.urihttps://hdl.handle.net/10316/104862-
dc.description.abstractNeuroblastoma (NB) represents the most frequent and aggressive form of extracranial solid tumor of infants. Nucleolin (NCL) is a protein overexpressed and partially localized on the cell surface of tumor cells of adult cancers. Little is known about NCL and pediatric tumors and nothing is reported about cell surface NCL and NB. Methods: NB cell lines, Schwannian stroma-poor NB tumors and bone marrow (BM)-infiltrating NB cells were evaluated for the expression of cell surface NCL by Flow Cytometry, Imaging Flow Cytometry and Immunohistochemistry analyses. The cytotoxic activity of doxorubicin (DXR)-loaded nanocarriers decorated with the NCL-recognizing F3 peptide (T-DXR) was evaluated in terms of inhibition of NB cell proliferation and induction of cell death in vitro, whereas metastatic and orthotopic animal models of NB were used to examine their in vivo antitumor potential. Results: NB cell lines, NB tumor cells (including patient-derived and Patient-Derived Xenografts-PDX) and 70% of BM-infiltrating NB cells show cell surface NCL expression. NCL staining was evident on both tumor and endothelial tumor cells in NB xenografts. F3 peptide-targeted nanoparticles, co-localizing with cell surface NCL, strongly associates with NB cells showing selective tumor cell internalization. T-DXR result significantly more effective, in terms of inhibition of cell proliferation and reduction of cell viability in vitro, and in terms of delay of tumor growth in all NB animal model tested, when compared to both control mice and those treated with the untargeted formulation. Conclusions: Our findings demonstrate that NCL could represent an innovative therapeutic cellular target for NB.pt
dc.language.isoengpt
dc.publisherSpringer Naturept
dc.relationItalian ministry of Health under the frame of EuroNanoMed II-2015 (ER-2015- 2360441-Eranet to F.P.) and Associazione Italiana per la Ricerca sul Cancro, Investigator Grant n. 18474 to M.P. and n. 24397 to F.Ppt
dc.relationCENTRO-01-0247-FEDER-017646 (ODD4PEGASEMP)pt
dc.relationPOCI-01-0145-FEDER- 016390pt
dc.relationEuronanomed (FCT reference ENMed/0005/2015)pt
dc.relationCENT RO-01-0145-FEDER-000012-HealthyAging2020pt
dc.relationUIDB/ 04539/2020pt
dc.relationItalian Foundation for Neuroblastoma research and Associazione Oncologia Pediatrica E Neuroblastoma (OPEN) ONLUSpt
dc.rightsopenAccesspt
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt
dc.subjectNeuroblastomapt
dc.subjectCell surface proteinpt
dc.subjectNucleolinpt
dc.subjectTargeted therapypt
dc.subjectNanotechnologypt
dc.subject.meshAnimalspt
dc.subject.meshAntineoplastic Agentspt
dc.subject.meshBone Marrow Cellspt
dc.subject.meshCell Line, Tumorpt
dc.subject.meshCell Membranept
dc.subject.meshCell Proliferationpt
dc.subject.meshCell Survivalpt
dc.subject.meshDoxorubicinpt
dc.subject.meshHeterograftspt
dc.subject.meshHumanspt
dc.subject.meshLiposomespt
dc.subject.meshMicept
dc.subject.meshNanoparticlespt
dc.subject.meshNeuroblastomapt
dc.subject.meshPeptidespt
dc.subject.meshPhosphoproteinspt
dc.subject.meshRNA-Binding Proteinspt
dc.titleCell surface Nucleolin represents a novel cellular target for neuroblastoma therapypt
dc.typearticle-
degois.publication.firstPage180pt
degois.publication.issue1pt
degois.publication.titleJournal of Experimental and Clinical Cancer Researchpt
dc.peerreviewedyespt
dc.identifier.doi10.1186/s13046-021-01993-9pt
degois.publication.volume40pt
dc.date.embargo2021-06-02*
uc.date.periodoEmbargo0pt
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypearticle-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.fulltextCom Texto completo-
item.languageiso639-1en-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.orcid0000-0002-3404-5755-
crisitem.author.orcid0000-0001-5820-9964-
crisitem.author.orcid0000-0003-3449-0522-
Appears in Collections:I&D CNC - Artigos em Revistas Internacionais
I&D CIBB - Artigos em Revistas Internacionais
FFUC- Artigos em Revistas Internacionais
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