Utilize este identificador para referenciar este registo:
https://hdl.handle.net/10316/103763
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Marques, Ana P. | - |
dc.contributor.author | Resende, Rosa | - |
dc.contributor.author | Silva, Diana F. | - |
dc.contributor.author | Batista, Mariana | - |
dc.contributor.author | Pereira, Daniela | - |
dc.contributor.author | Wildenberg, Brigite | - |
dc.contributor.author | Morais, Sofia | - |
dc.contributor.author | Macedo, António | - |
dc.contributor.author | Pais, Claudia | - |
dc.contributor.author | Melo, Joana B. | - |
dc.contributor.author | Madeira, Nuno | - |
dc.contributor.author | Pereira, Cláudia F. | - |
dc.date.accessioned | 2022-11-25T10:52:42Z | - |
dc.date.available | 2022-11-25T10:52:42Z | - |
dc.date.issued | 2021-05-07 | - |
dc.identifier.issn | 2227-9059 | - |
dc.identifier.uri | https://hdl.handle.net/10316/103763 | - |
dc.description.abstract | This study aims to evaluate whether mitochondrial changes occur in the early stages of bipolar disorder (BD). Using fibroblasts derived from BD patients and matched controls, the levels of proteins involved in mitochondrial biogenesis and dynamics (fission and fusion) were evaluated by Western Blot analysis. Mitochondrial membrane potential (MMP) was studied using the fluorescent probe TMRE. Mitochondrial morphology was analyzed with the probe Mitotracker Green and mitophagy was evaluated by quantifying the co-localization of HSP60 (mitochondria marker) and LC3B (autophagosome marker) by immunofluorescence. Furthermore, the activity of the mitochondrial respiratory chain and the glycolytic capacity of controls and BD patients-derived cells were also studied using the Seahorse technology. BD patient-derived fibroblasts exhibit fragmented mitochondria concomitantly with changes in mitochondrial dynamics and biogenesis in comparison with controls. Moreover, a decrease in the MMP and increased mitophagy was observed in fibroblasts obtained from BD patients when compared with control cells. Impaired energetic metabolism due to inhibition of the mitochondrial electron transport chain (ETC) and subsequent ATP depletion, associated with glycolysis stimulation, was also a feature of BD fibroblasts. Overall, these results support the fact that mitochondrial disturbance is an early event implicated in BD pathophysiology that might trigger neuronal changes and modification of brain circuitry. | pt |
dc.language.iso | eng | pt |
dc.publisher | MDPI AG | pt |
dc.relation | CENTRO-01-0145-FEDER-000012 (HealthyAging2020) | pt |
dc.relation | POCI-01-0145-FEDER-028214 (MAM4BD) | pt |
dc.relation | UIDB/04539/2020 | pt |
dc.relation | European Social Fund - Post-Doctoral Researcher Contract SFRH/BPD/101028/2014 | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject | bipolar disorder | pt |
dc.subject | mitochondrial dysfunction | pt |
dc.subject | mitochondrial biogenesis | pt |
dc.subject | mitophagy | pt |
dc.subject | bioenergetics | pt |
dc.subject | fibroblasts | pt |
dc.title | Mitochondrial Alterations in Fibroblasts of Early Stage Bipolar Disorder Patients | pt |
dc.type | article | pt |
degois.publication.firstPage | 522 | pt |
degois.publication.issue | 5 | pt |
degois.publication.title | Biomedicines | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.3390/biomedicines9050522 | - |
degois.publication.volume | 9 | pt |
dc.date.embargo | 2021-05-07 | * |
dc.identifier.pmid | 34066918 | - |
uc.date.periodoEmbargo | 0 | pt |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.project.grantno | Center for Innovative Biomedicine and Biotechnology - CIBB | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0002-0504-5756 | - |
crisitem.author.orcid | 0000-0002-0612-2645 | - |
crisitem.author.orcid | 0000-0002-0249-1603 | - |
crisitem.author.orcid | 0000-0003-2180-2718 | - |
crisitem.author.orcid | 0000-0001-5049-2670 | - |
crisitem.author.orcid | 0000-0001-5009-8841 | - |
crisitem.author.orcid | 0000-0002-6630-5056 | - |
Aparece nas coleções: | I&D CIBB - Artigos em Revistas Internacionais I&D CNC - Artigos em Revistas Internacionais IIIUC - Artigos em Revistas Internacionais FMUC Medicina - Artigos em Revistas Internacionais I&D CIBIT - Artigos em Revistas Internacionais I&D ICBR - Artigos em Revistas Internacionais |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
Mitochondrial-alterations-in-fibroblasts-of-early-stage-bipolar-disorder-patientsBiomedicines.pdf | 1.89 MB | Adobe PDF | Ver/Abrir |
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