Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/103763
DC FieldValueLanguage
dc.contributor.authorMarques, Ana P.-
dc.contributor.authorResende, Rosa-
dc.contributor.authorSilva, Diana F.-
dc.contributor.authorBatista, Mariana-
dc.contributor.authorPereira, Daniela-
dc.contributor.authorWildenberg, Brigite-
dc.contributor.authorMorais, Sofia-
dc.contributor.authorMacedo, António-
dc.contributor.authorPais, Claudia-
dc.contributor.authorMelo, Joana B.-
dc.contributor.authorMadeira, Nuno-
dc.contributor.authorPereira, Cláudia F.-
dc.date.accessioned2022-11-25T10:52:42Z-
dc.date.available2022-11-25T10:52:42Z-
dc.date.issued2021-05-07-
dc.identifier.issn2227-9059-
dc.identifier.urihttps://hdl.handle.net/10316/103763-
dc.description.abstractThis study aims to evaluate whether mitochondrial changes occur in the early stages of bipolar disorder (BD). Using fibroblasts derived from BD patients and matched controls, the levels of proteins involved in mitochondrial biogenesis and dynamics (fission and fusion) were evaluated by Western Blot analysis. Mitochondrial membrane potential (MMP) was studied using the fluorescent probe TMRE. Mitochondrial morphology was analyzed with the probe Mitotracker Green and mitophagy was evaluated by quantifying the co-localization of HSP60 (mitochondria marker) and LC3B (autophagosome marker) by immunofluorescence. Furthermore, the activity of the mitochondrial respiratory chain and the glycolytic capacity of controls and BD patients-derived cells were also studied using the Seahorse technology. BD patient-derived fibroblasts exhibit fragmented mitochondria concomitantly with changes in mitochondrial dynamics and biogenesis in comparison with controls. Moreover, a decrease in the MMP and increased mitophagy was observed in fibroblasts obtained from BD patients when compared with control cells. Impaired energetic metabolism due to inhibition of the mitochondrial electron transport chain (ETC) and subsequent ATP depletion, associated with glycolysis stimulation, was also a feature of BD fibroblasts. Overall, these results support the fact that mitochondrial disturbance is an early event implicated in BD pathophysiology that might trigger neuronal changes and modification of brain circuitry.pt
dc.language.isoengpt
dc.publisherMDPI AGpt
dc.relationCENTRO-01-0145-FEDER-000012 (HealthyAging2020)pt
dc.relationPOCI-01-0145-FEDER-028214 (MAM4BD)pt
dc.relationUIDB/04539/2020pt
dc.relationEuropean Social Fund - Post-Doctoral Researcher Contract SFRH/BPD/101028/2014pt
dc.rightsopenAccesspt
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt
dc.subjectbipolar disorderpt
dc.subjectmitochondrial dysfunctionpt
dc.subjectmitochondrial biogenesispt
dc.subjectmitophagypt
dc.subjectbioenergeticspt
dc.subjectfibroblastspt
dc.titleMitochondrial Alterations in Fibroblasts of Early Stage Bipolar Disorder Patientspt
dc.typearticlept
degois.publication.firstPage522pt
degois.publication.issue5pt
degois.publication.titleBiomedicinespt
dc.peerreviewedyespt
dc.identifier.doi10.3390/biomedicines9050522-
degois.publication.volume9pt
dc.date.embargo2021-05-07*
dc.identifier.pmid34066918-
uc.date.periodoEmbargo0pt
item.openairetypearticle-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.fulltextCom Texto completo-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.orcid0000-0002-0504-5756-
crisitem.author.orcid0000-0002-0612-2645-
crisitem.author.orcid0000-0002-0249-1603-
crisitem.author.orcid0000-0003-2180-2718-
crisitem.author.orcid0000-0001-5009-8841-
crisitem.author.orcid0000-0002-6630-5056-
Appears in Collections:I&D CIBB - Artigos em Revistas Internacionais
I&D CNC - Artigos em Revistas Internacionais
IIIUC - Artigos em Revistas Internacionais
FMUC Medicina - Artigos em Revistas Internacionais
I&D CIBIT - Artigos em Revistas Internacionais
I&D ICBR - Artigos em Revistas Internacionais
Show simple item record

Google ScholarTM

Check

Altmetric

Altmetric


This item is licensed under a Creative Commons License Creative Commons