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Title: Open-Air Cold Plasma Device Leads to Selective Tumor Cell Cytotoxicity
Authors: Almeida-Ferreira, Catarina 
Silva-Teixeira, Rafael 
Laranjo, Mafalda 
Almeida, Nuno 
Brites, Gonçalo 
Dias-Ferreira, João 
Marques, Inês A. 
Neves, Rita 
Serambeque, Beatriz 
Teixo, Ricardo 
Abrantes, Ana Margarida 
Caramelo, Francisco 
Botelho, Maria Filomena 
Keywords: cancer; cold atmospheric plasma; neoplasms (MESH); plasma gases (MESH)
Issue Date: 2021
Publisher: MDPI
Serial title, monograph or event: Applied Sciences (Switzerland)
Volume: 11
Issue: 9
Abstract: The need for effective and safe therapies for cancer is growing as aging is modifying its epidemiology. Cold atmospheric plasma (CAP) has gained attention as a potential anti-tumor therapy. CAP is a gas with enough energy to ionize a significant fraction of its constituent particles, forming equal numbers of positive ions and electrons. Timely-resolved output voltage measurement, emission spectroscopy, and quantification of reactive species (RS) in plasma-activated media (PAM) were performed to characterize the physical and chemical properties of plasma. To assess the cytotoxicity of cold atmospheric plasma in human tumors, different cell lines were cultured, plated, and exposed to CAP, followed by MTT and SRB colorimetric assays 24 h later. Human fibroblasts, phenotypically normal cells, were processed similarly. Plasma cytotoxicity was higher in cells of breast cancer, urinary bladder cancer, osteosarcoma, lung cancer, melanoma, and endometrial cancer. Cytotoxicity was time-dependent and possibly related to the increased production of hydrogen peroxide in the exposed medium. Sixty seconds of CAP exposure renders selective effects, preserving the viability of fibroblast cells. These results point to the importance of conducting further studies of the therapy with plasma.
ISSN: 2076-3417
DOI: 10.3390/app11094171
Rights: openAccess
Appears in Collections:FMUC Medicina - Artigos em Revistas Internacionais
I&D ICBR - Artigos em Revistas Internacionais
I&D CIBB - Artigos em Revistas Internacionais

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