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Title: Isolation and Identification of Cytotoxic Compounds Present in Biomaterial Life®
Authors: Ferreira, Maria Beatriz 
Pereira, Nelson A. M. 
Marto, Carlos Miguel 
Cardoso, Miguel 
Amaro, Inês 
Coelho, Ana 
Saraiva, José 
Spagnuolo, Gianrico
Ferreira, Manuel Marques 
Piñeiro, Marta 
Melo, T. M. V. D. Pinho e 
Botelho, Maria Filomena 
Carrilho, Eunice 
Paula, Anabela 
Laranjo, Mafalda 
Keywords: direct pulp capping; biomaterial; calcium hydroxide; cytotoxicity; odontoblasts
Issue Date: 24-Jan-2022
Project: UID/NEU/04539/2019 
Serial title, monograph or event: Materials
Volume: 15
Issue: 3
Abstract: Direct pulp capping consists of a procedure in which a material is directly placed over the exposed pulp to maintain dental vitality. Although still widely used in clinical practice, previous in vitro studies found that the biomaterial Life® presented high cytotoxicity, leading to cell death. This study aimed to identify the Life® constituents responsible for its cytotoxic effects on odontoblast-like cells (MDPC-23). Aqueous medium conditioned with Life® was subjected to liquid-liquid extraction with ethyl acetate. After solvent removal, cells were treated with residues isolated from the organic and aqueous fractions. MTT and Trypan blue assays were carried out to evaluate the metabolic activity and cell death. The organic phase residue promoted a significant decrease in metabolic activity and increased cell death. On the contrary, no cytotoxic effects were observed with the mixture from the aqueous fraction. Spectroscopic and spectrometric methods allowed the identification of the toxic compounds. A mixture of the regioisomers ortho, para, and meta of N-ethyl-toluenesulfonamide was identified as the agent responsible for the toxicity of biomaterial Life® in MDPC-23 cells. These findings contribute to improving biomaterial research and development.
ISSN: 1996-1944
DOI: 10.3390/ma15030871
Rights: openAccess
Appears in Collections:FMUC Medicina - Artigos em Revistas Internacionais
I&D CQC - Artigos em Revistas Internacionais
I&D ICBR - Artigos em Revistas Internacionais
I&D CIBB - Artigos em Revistas Internacionais

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