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Title: Clenbuterol Storage Stability in the Bovine Urine and Liver Samples Used for European Official Control in the Azores Islands (Portugal)
Authors: Pinheiro, Isabel 
Jesuíno, Bruno 
Barbosa, Jorge 
Ferreira, Humberto 
Ramos, Fernando 
Matos, José 
Silveira, Maria Irene Noronha da 
Keywords: Clenbuterol; Urine; Liver; Stability; GC-MS
Issue Date: 11-Feb-2009
Publisher: American Chemical Society
Citation: Journal of Agricultural and Food Chemistry. 57:3 (2009) 910-914
Abstract: Clenbuterol is a well-known growth promoter, illegally used in farm animals, especially in cattle. Samples collected for the screening of β2-agonist residues in Portuguese Azores Islands must travel through all the nine islands until they reach Azores Central Laboratory. If any suspicious sample is detected, it must be further transported to the National Reference Laboratory in Lisbon for confirmation. As a consequence of these circumstances, samples are submitted to different transport and storage times, as well as different temperature conditions and in some cases successive freezing and thawing cycles. As clenbuterol is the most detected β2-agonist growth promoter in the Portuguese Residue Monitoring Plan, studies were conducted on the stability of this compound in incurred samples (bovine liver and urine) at +4, −20 and −60 °C over time. Samples kept at −20 °C were also analyzed over time after successive freezing and thawing cycles. The analyses of clenbuterol over time were performed by gas chromatography−mass spectrometry (GC−MS) with selected ion monitoring (SIM). Clenbuterol in incurred urine and liver samples was significantly stable up to 20 weeks at −20 and −60 °C and after, at least, six consecutive freezings and thawings. At +4 °C, clenbuterol remained stable, at least until 12 weeks in urine and up to 20 weeks in liver.
ISSN: 0021-8561
DOI: 10.1021/jf802995e
Rights: openAccess
Appears in Collections:FFUC- Artigos em Revistas Internacionais

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