Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/100827
DC FieldValueLanguage
dc.contributor.authorPereira, Cátia-
dc.contributor.authorMontalbán, Itziar A.-
dc.contributor.authorPedrosa, Ana-
dc.contributor.authorTavares, Jéssica-
dc.contributor.authorPestryakov, Alexey-
dc.contributor.authorBogdanchikova, Nina-
dc.contributor.authorCanhoto, Jorge-
dc.contributor.authorMoncaleán, Paloma-
dc.date.accessioned2022-07-13T11:33:50Z-
dc.date.available2022-07-13T11:33:50Z-
dc.date.issued2021-
dc.identifier.issn1999-4907pt
dc.identifier.urihttp://hdl.handle.net/10316/100827-
dc.description.abstractOrganogenesis and somatic embryogenesis have been widely applied as the two main regeneration pathways in plant tissue cultures. However, recalcitrance is still the main restriction in the clonal propagation of many woody species, especially in conifers. They undergo a “phase change” that leads to significant loss of vegetative propagation capacity, reducing the aptitude of tissues and organs to be regenerated in vitro beyond this point. In line with this, the in vitro regeneration of mature conifer trees has been a long-cherished goal in many laboratories worldwide. Based on previous works in Pinus species regeneration from adult trees, we now present data about the culture of apical shoot buds in an attempt to induce organogenesis and somatic embryogenesis to clone mature trees of Aleppo pine (Pinus halepensis). Reinvigorated axillary shoots were submitted to conditions usually applied to induce somatic embryogenesis through the manipulation of culture media, including the use of auxins such as 2,4-Dichlorophenoxyacetic acid and 1-Naphthaleneacetic acid, cytokinins (6-benzyladenine and kinetin), and phytosulfokine (50, 100, and 200 nM). Although somatic embryos could not be obtained, an embryogenic-like tissue was produced, followed by the emergence of actively proliferating non-embryogenic calli. Variations in the consistence, texture, and color of non-embryogenic calli were observed; especially those arising in the media containing phytosulfokine. Reinvigorated shoots, induced by 22 or 44 M 6-benzyladenine, were obtained through organogenesis and acclimatized, and phenotypically normal plants were obtained.pt
dc.language.isoengpt
dc.relationMINECO (Spanish Government) project (AGL2016-76143-C4-3R)pt
dc.relationBIOALI-CYTED (P117RT0522),pt
dc.relationDECO (Basque government, Ayudas de formación a jóvenes investigadores y tecnólogos)pt
dc.relationRenature: Projecto ReNature (Centro-01-0145-FEDER-000007)pt
dc.relationFCT SFRH/BD/123702/2016pt
dc.relationTomsk Polytechnic University Competitiveness Enhancement Program project VIU-ISHBMT-197/2020pt
dc.relationEuropean Union’s Horizon 2020 research and innovation program, under agreement Nº 773324pt
dc.relationFCT/MCTES UIDB/04004/2020pt
dc.rightsopenAccesspt
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt
dc.subjectAleppo pinept
dc.subjectconiferspt
dc.subjectphytosulfokinept
dc.subjectplant growth regulatorspt
dc.subjectrootingpt
dc.titleRegeneration of Pinus halepensis (Mill.) through Organogenesis from Apical Shoot Budspt
dc.typearticle-
degois.publication.firstPage363pt
degois.publication.issue3pt
degois.publication.titleForestspt
dc.peerreviewedyespt
dc.identifier.doi10.3390/f12030363pt
degois.publication.volume12pt
dc.date.embargo2021-01-01*
uc.date.periodoEmbargo0pt
item.grantfulltextopen-
item.fulltextCom Texto completo-
item.languageiso639-1en-
crisitem.author.deptFaculty of Sciences and Technology-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.researchunitCFE - Centre for Functional Ecology - Science for People & the Planet-
crisitem.author.researchunitCFE - Centre for Functional Ecology - Science for People & the Planet-
crisitem.author.researchunitCFE - Centre for Functional Ecology - Science for People & the Planet-
crisitem.author.orcid0000-0001-8390-4774-
crisitem.author.orcid0000-0003-2299-298X-
Appears in Collections:I&D CFE - Artigos em Revistas Internacionais
Show simple item record

Google ScholarTM

Check

Altmetric

Altmetric


This item is licensed under a Creative Commons License Creative Commons