Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/100575
DC Field | Value | Language |
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dc.contributor.author | Abreu, Ricardo C de | - |
dc.contributor.author | Ramos, Cristiana V | - |
dc.contributor.author | Becher, Clarissa | - |
dc.contributor.author | Lino, Miguel | - |
dc.contributor.author | Jesus, Carlos | - |
dc.contributor.author | Martins, Paula A da Costa | - |
dc.contributor.author | Martins, Patrícia A T | - |
dc.contributor.author | Moreno, Maria João | - |
dc.contributor.author | Fernandes, Hugo | - |
dc.contributor.author | Ferreira, Lino | - |
dc.date.accessioned | 2022-07-03T16:32:04Z | - |
dc.date.available | 2022-07-03T16:32:04Z | - |
dc.date.issued | 2021-08 | - |
dc.identifier.issn | 2001-3078 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/100575 | - |
dc.description.abstract | Small extracellular vesicles (sEVs), through their natural ability to interact with biological membranes and exploit endogenous processing pathways to convey biological information, are quintessential for the delivery of therapeutically relevant compounds, such as microRNAs (miRNAs) and proteins. Here, we used a fluorescently-labelled miRNA to quantify the efficiency of different methods to modulate the cargo of sEVs. Our results showed that, compared with electroporation, heat shock, permeation by a detergent-based compound (saponin) or cholesterol-modification of the miRNA, Exo-Fect was the most efficient method with > 50% transfection efficiency. Furthermore, qRT-PCR data showed that, compared with native sEVs, Exo-Fect modulation led to a > 1000-fold upregulation of the miRNA of interest. Importantly, this upregulation was observed for sEVs isolated from multiple sources. The modulated sEVs were able to delivery miR-155-5p into a reporter cell line, confirming the successful delivery of the miRNA to the target cell and, more importantly, its functionality. Finally, we showed that the membrane of Exo-Fect-loaded sEVs was altered compared with native sEVs and that enhanced the internalization of Exo-Fect-loaded sEVs within the target cells and decreased the interaction of those modulated sEVs with lysosomes. | pt |
dc.language.iso | eng | pt |
dc.relation | 007630 UID/QUI/00313/2019 | pt |
dc.relation | PT2020_PTDC_DTP-FTO_2784_2014 | pt |
dc.relation | POCI-01-0145-FEDER-029919 | pt |
dc.relation | COMPETE2020- UE | pt |
dc.relation | CENTRO-01-0145-FEDER-000014 | pt |
dc.relation | Programa Operacional Regional do Centro’ CENTRO2020 | pt |
dc.relation | Projects Interreg entitled: ‘Impulso de una red de I+i en química biológica para diagnóstico y tratamiento de enfermedades neurológicas | pt |
dc.relation | EAPA_791/2018 - NEUROATLANTIC entitled: ‘An Atlantic innovation platform on diagnosis and treatment of neurological diseases and aging’ | pt |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/952266/EU/RESEarch for healThy AGEING | pt |
dc.rights | openAccess | pt |
dc.subject | extracellular vesicles; microRNA; modulation; post‐isolation | pt |
dc.subject.mesh | Cell Line | pt |
dc.subject.mesh | Drug Delivery Systems | pt |
dc.subject.mesh | Extracellular Vesicles | pt |
dc.subject.mesh | Genetic Vectors | pt |
dc.subject.mesh | HEK293 Cells | pt |
dc.subject.mesh | Humans | pt |
dc.subject.mesh | MicroRNAs | pt |
dc.subject.mesh | Microscopy, Electron, Transmission | pt |
dc.subject.mesh | Gene Transfer Techniques | pt |
dc.title | Exogenous loading of miRNAs into small extracellular vesicles | pt |
dc.type | article | - |
degois.publication.firstPage | e12111 | pt |
degois.publication.issue | 10 | pt |
degois.publication.title | J Extracell Vesicles | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.1002/jev2.12111 | pt |
degois.publication.volume | 10 | pt |
dc.date.embargo | 2021-08-01 | * |
uc.date.periodoEmbargo | 0 | pt |
item.grantfulltext | open | - |
item.fulltext | Com Texto completo | - |
item.openairetype | article | - |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
Appears in Collections: | UC Bibliotecas - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Manuscript_with_Figures.pdf | 6.38 MB | Adobe PDF | View/Open |
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