This research was developed in the scope of the Project GENIALG “GENetic diversity exploitation for Innovative macro-ALGal biorefinery” with funding from the European Union’s Horizon 2020 Framework Program under grant agreement No 727892, and the project PTDC/QUIOUT/ 29373/2017 (FightSterol) with funding from national funds (OE) and co-financed by the FEDER, by the Operational Program of Competitiveness and Internationalization (POCI), within the PT2020 Partnership Agreement. The authors also acknowledge to FCT/MCT (Portugal) for the financial support of LAQV-REQUIMTE (UIDB/50006/2020+UIDP/50006/2020), CICECO(UIDB/50011/2020+UIDP /50011/2020+LA/P/006/2020), CESAM (UIDP/50017/2020 +UIDB/50017/2020+LA/P/0094/2020) and RNEM—Portuguese Mass Spectrometry Network (LISBOA-01-0145-FEDER-402-022125), through national funds and, where applicable, the co-funding by the FEDER, within the PT2020 Partnership Agreement and Compete 2020. NMR data were collected at the UC-NMR facility which is supported in part by FEDER—European Regional Development Fund through the COMPETE Programme (Operational Programme for Competitiveness) and by National Funds through FCT through grants RECI/QEQ-QFI/0168/2012, CENTRO-07-CT62-FEDER-002012, and also through support to Rede Nacional de Ressonância Magnética Nuclear (RNRMN) and to Coimbra Chemistry Centre through grant UID/QUI/00313/2019. Cláudia Nunes thanks national funds (OE), through FCT, I.P., in the scope of the framework contract foreseen in the numbers 4, 5, and 6 of article 23, of the Decree-Law 57/2016, of August 29, changed by Law 57/2017, of July 19. Filipe M. Coreta-Gomes, Ana S. P. Moreira and Sónia S. Ferreira would like to acknowledge the research contract funding from the LAQV-REQUIMTE (UIDB/50006/2020) project.