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Title: Olive Varieties under UV-B Stress Show Distinct Responses in Terms of Antioxidant Machinery and Isoform/Activity of RubisCO
Authors: Piccini, Chiara
Cai, Giampiero
Dias, Maria Celeste 
Araújo, Márcia 
Parri, Sara
Romi, Marco
Faleri, Claudia
Cantini, Claudio
Keywords: Antioxidant enzymes; Heat shock proteins; Olea europaea; RubisCO; Sucrose synthase; UV-B radiation
Issue Date: 2021
Project: SFRH/BD/116801/2016 
Serial title, monograph or event: International Journal of Molecular Sciences
Volume: 22
Issue: 20
Abstract: In recent decades, atmospheric pollution led to a progressive reduction of the ozone layer with a consequent increase in UV-B radiation. Despite the high adaptation of olive trees to the Mediterranean environment, the progressive increase of UV-B radiation is a risk factor for olive tree cultivation. It is therefore necessary to understand how high levels of UV-B radiation affect olive plants and to identify olive varieties which are better adapted. In this study we analyzed two Italian olive varieties subjected to chronic UV-B stress. We focused on the effects of UV-B radiation on RubisCO, in terms of quantity, enzymatic activity and isoform composition. In addition, we also analyzed changes in the activity of antioxidant enzymes (SOD, CAT, GPox) to get a comprehensive picture of the antioxidant system. We also evaluated the effects of UV-B on the enzyme sucrose synthase. The overall damage at biochemical level was also assessed by analyzing changes in Hsp70, a protein triggered under stress conditions. The results of this work indicate that the varieties (Giar-raffa and Olivastra Seggianese) differ significantly in the use of specific antioxidant defense systems, as well as in the activity and isoform composition of RubisCO. Combined with a different use of sucrose synthase, the overall picture shows that Giarraffa optimized the use of GPox and opted for a targeted choice of RubisCO isoforms, in addition to managing the content of sucrose synthase, thereby saving energy during critical stress points. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
ISSN: 1422-0067
DOI: 10.3390/ijms222011214
Rights: openAccess
Appears in Collections:I&D CFE - Artigos em Revistas Internacionais

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