Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/95820
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dc.contributor.authorGonçalves, Ana Cristina-
dc.contributor.authorAlves, Raquel-
dc.contributor.authorBaldeiras, Inês-
dc.contributor.authorMarques, Bárbara-
dc.contributor.authorOliveiros, Bárbara-
dc.contributor.authorPereira, Amélia-
dc.contributor.authorCosta, José Manuel Nascimento-
dc.contributor.authorCortesão, Emília-
dc.contributor.authorVieira, Luísa Mota-
dc.contributor.authorRibeiro, Ana Bela Sarmento-
dc.date.accessioned2021-09-27T16:20:28Z-
dc.date.available2021-09-27T16:20:28Z-
dc.date.issued2021-06-23-
dc.identifier.issn2072-6694pt
dc.identifier.urihttp://hdl.handle.net/10316/95820-
dc.description.abstractOxidative stress and abnormal DNA methylation have been implicated in cancer, including myelodysplastic syndromes (MDSs). This fact leads us to investigate whether oxidative stress is correlated with localized and global DNA methylations in the peripheral blood of MDS patients. Sixty-six MDS patients and 26 healthy individuals were analyzed. Several oxidative stress and macromolecule damage parameters were analyzed. Localized (gene promotor) and global DNA methylations (5-mC and 5-hmC levels; LINE-1 methylation) were assessed. MDS patients had lower levels of reduced glutathione and total antioxidant status (TAS) and higher levels of peroxides, nitric oxide, peroxides/TAS, and 8-hydroxy-2-deoxyguanosine compared with controls. These patients had higher 5-mC levels and lower 5-hmC/5-mC ratio and LINE-1 methylation and increased methylation frequency of at least one methylated gene. Peroxide levels and peroxide/TAS ratio were higher in patients with methylated genes than those without methylation and negatively correlated with LINE-1 methylation and positively with 5-mC levels. The 5-hmC/5-mC ratio was significantly associated with progression to acute leukemia and peroxide/TAS ratio with overall survival. This study points to a relationship between oxidative stress and DNA methylation, two common pathogenic mechanisms involved in MDS, and suggests the relevance of 5-hmC/5-mC and peroxide/TAS ratios as complementary prognostic biomarkers.pt
dc.description.sponsorshipThe present work was supported by CIMAGO (Center of Investigation on Environment Genetics and Oncobiology), Faculty of Medicine, University of Coimbra, Portugal; by a grant from Santander-Totta Bank/Gabinete de Apoio/Investigação (GAI) of the Faculty of Medicine, University of Coimbra, Portugal; and by the Foundation for Science and Technology (FCT), Portugalpt
dc.language.isoengpt
dc.publisherMDPIpt
dc.relationUID/NEU/04539/2019pt
dc.relationUIDB/04539/2020pt
dc.relationUIDP/04539/2020pt
dc.rightsopenAccesspt
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt
dc.subjectDNA methylationpt
dc.subjectAcute myeloid leukemiapt
dc.subjectBlood biomarkerspt
dc.subjectMyelodysplastic syndromept
dc.subjectOxidative stresspt
dc.subjectPrognosispt
dc.subjectProgressionpt
dc.subjectSurvivalpt
dc.titleDNA Methylation Is Correlated with Oxidative Stress in Myelodysplastic Syndrome-Relevance as Complementary Prognostic Biomarkerspt
dc.typearticle-
degois.publication.firstPage3138pt
degois.publication.issue13pt
degois.publication.titleCancerspt
dc.peerreviewedyespt
dc.identifier.doi10.3390/cancers13133138pt
degois.publication.volume13pt
dc.date.embargo2021-06-23*
uc.date.periodoEmbargo0pt
item.grantfulltextopen-
item.languageiso639-1en-
item.fulltextCom Texto completo-
crisitem.author.deptFaculty of Medicine-
crisitem.author.deptFaculty of Medicine-
crisitem.author.deptFaculty of Medicine-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.orcid0000-0001-7836-8161-
crisitem.author.orcid0000-0002-4142-4841-
Appears in Collections:FMUC Medicina - Artigos em Revistas Internacionais
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