Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/95310
DC Field | Value | Language |
---|---|---|
dc.contributor.author | DiNunzio, Giada | - |
dc.contributor.author | Belew, Getachew D. | - |
dc.contributor.author | Torres, Alejandra N. | - |
dc.contributor.author | Silva, Joao Gabriel | - |
dc.contributor.author | Silva, Luís P. | - |
dc.contributor.author | Barosa, Cristina | - |
dc.contributor.author | Tavares, Ludgero | - |
dc.contributor.author | Jones, John G. | - |
dc.date.accessioned | 2021-07-12T08:11:01Z | - |
dc.date.available | 2021-07-12T08:11:01Z | - |
dc.date.issued | 2020-12 | - |
dc.identifier.issn | 2045-2322 | pt |
dc.identifier.other | 2-s2.0-85088824549 | en_US |
dc.identifier.uri | https://hdl.handle.net/10316/95310 | - |
dc.description.abstract | Excessive sugar intake including high-fructose corn syrup (HFCS) is implicated in the rise of obesity, insulin resistance and non-alcoholic fatty liver disease. Liver glycogen synthesis is influenced by both fructose and insulin signaling. Therefore, the effect of HFCS on hepatic glycogenesis was evaluated in mice feeding ad-libitum. Using deuterated water: the fraction of glycogen derived from triose-P sources, Krebs cycle substrates, and direct pathway + cycling, was measured in 9 normal-chow fed mice (NC) and 12 mice fed normal chow plus a 55% fructose/45% glucose mix in the drinking water at 30% w/v (HFCS-55). This was enriched with [U-13C]fructose or [U-13C]glucose to determine the contribution of each to glycogenesis. For NC, direct pathway + cycling, Krebs cycle, and triose-P sources accounted for 66 ± 0.7%, 23 ± 0.8% and 11 ± 0.4% of glycogen synthesis, respectively. HFCS-55 mice had similar direct pathway + cycling (64 ± 1%) but lower Krebs cycle (12 ± 1%, p < 0.001) and higher triose-P contributions (24 ± 1%, p < 0.001). HFCS-55-fructose contributed 17 ± 1% via triose-P and 2 ± 0% via Krebs cycle. HFCS-55-glucose contributed 16 ± 3% via direct pathway and 1 ± 0% via Krebs cycle. In conclusion, HFCS-55 supplementation resulted in similar hepatic glycogen deposition rates. Indirect pathway contributions shifted from Krebs cycle to Triose-P sources reflecting HFCS-55-fructose utilization, while HFCS-55-glucose was incorporated almost exclusively by the direct pathway. | eng |
dc.language.iso | eng | pt |
dc.publisher | Nature | pt |
dc.relation | Marie Sklodowska-Curie Grant Agreement No. 722619 (FOIE GRAS) | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.title | Determining the contribution of a high-fructose corn syrup formulation to hepatic glycogen synthesis during ad-libitum feeding in mice | eng |
dc.type | article | en_US |
degois.publication.issue | 12852 | pt |
degois.publication.title | Scientific Reports | pt |
dc.date.updated | 2021-07-09T23:33:03Z | - |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.1038/s41598-020-69820-3 | pt |
degois.publication.volume | 10 | pt |
dc.description.version | FB1F-B5BF-8A86 | Getachew Debas Belew | - |
dc.description.version | N/A | - |
dc.identifier.slug | cv-prod-2556392 | - |
dc.date.embargo | 2020-12-01 | * |
uc.date.periodoEmbargo | 0 | pt |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.openairetype | article | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.fulltext | Com Texto completo | - |
item.languageiso639-1 | en | - |
crisitem.project.grantno | info:eu-repo/grantAgreement/EC/H2020/722619/EU/Bioenergetic Remodeling in the Pathophysiology and Treatment of Non-Alcoholic Fatty Liver Disease | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0002-2324-1259 | - |
crisitem.author.orcid | 0000-0002-3745-3885 | - |
Appears in Collections: | I&D CNC - Artigos em Revistas Internacionais |
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File | Description | Size | Format | |
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Giada_et_al.,_2020_Sci.Reports.pdf | 1.5 MB | Adobe PDF | View/Open |
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