Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/92392
DC FieldValueLanguage
dc.contributor.authorPraça, Catarina-
dc.contributor.authorRosa, Susana C.-
dc.contributor.authorSevin, Emmanuel-
dc.contributor.authorCecchelli, Romeo-
dc.contributor.authorDehouck, Marie-Pierre-
dc.contributor.authorFerreira, Lino S.-
dc.date.accessioned2021-01-02T15:11:09Z-
dc.date.available2021-01-02T15:11:09Z-
dc.date.issued2019-
dc.identifier.issn22136711pt
dc.identifier.urihttps://hdl.handle.net/10316/92392-
dc.description.abstractThe derivation of human brain capillary endothelial cells is of utmost importance for drug discovery programs focusing on diseases of the central nervous system. Here, we describe a two-step differentiation protocol to derive brain capillary-like endothelial cells from human pluripotent stem cells. The cells were initially differentiated into endothelial progenitor cells followed by specification into a brain capillary-like endothelial cell phenotype using a protocol that combined the induction, in a time-dependent manner, of VEGF, Wnt3a, and retinoic acid signaling pathways and the use of fibronectin as the extracellular matrix. The brain capillary-like endothelial cells displayed a permeability to lucifer yellow of 1 × 10-3 cm/min, a transendothelial electrical resistance value of 60 Ω cm2 and were able to generate a continuous monolayer of cells expressing ZO-1 and CLAUDIN-5 but moderate expression of P-glycoprotein. Further maturation of these cells required coculture with pericytes. The study presented here opens a new approach for the study of soluble and non-soluble factors in the specification of endothelial progenitor cells into brain capillary-like endothelial cells.pt
dc.description.sponsorshipLF would like to thank the funding obtained by the Portugal2020 project StrokeTherapy (Project reference: 3386), EC project ERA chair (ERA@UC, ref. 669088) and FCT projects (PTDC/DTP-FTO/2784/2014 and 02/SAICT/2017/029229). CP and SR would like to thank the fellowships of FCT (SFRH/BD/51678/2011; SFRH/BPD/79323/2011, respectively).-
dc.language.isoengpt
dc.publisherElsevierpt
dc.rightsopenAccesspt
dc.subjectblood-brain barrier; brain capillary-like endothelial cells; extracellular matrices; induced pluripotent stem cells; in vitro model; soluble factorspt
dc.subject.meshBiomarkerspt
dc.subject.meshBlood-Brain Barrierpt
dc.subject.meshCells, Culturedpt
dc.subject.meshEndothelial Progenitor Cellspt
dc.subject.meshEndothelium, Vascularpt
dc.subject.meshExtracellular Matrixpt
dc.subject.meshHumanspt
dc.subject.meshImmunophenotypingpt
dc.subject.meshPluripotent Stem Cellspt
dc.subject.meshCell Differentiationpt
dc.titleDerivation of Brain Capillary-like Endothelial Cells from Human Pluripotent Stem Cell-Derived Endothelial Progenitor Cellspt
dc.typearticle-
degois.publication.firstPage599-611pt
degois.publication.lastPage611pt
degois.publication.issue4pt
degois.publication.titleStem Cell Reportspt
dc.peerreviewedyespt
dc.identifier.doi10.1016/j.stemcr.2019.08.002pt
degois.publication.volume13pt
dc.date.embargo2019-01-01*
uc.date.periodoEmbargo0pt
item.openairetypearticle-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.fulltextCom Texto completo-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.orcid0000-0001-8985-9302-
Appears in Collections:UC Bibliotecas - Artigos em Revistas Internacionais
Files in This Item:
File Description SizeFormat
Almeida Stem Cell Reports.pdf19.93 MBAdobe PDFView/Open
Show simple item record

SCOPUSTM   
Citations

22
checked on Nov 9, 2022

WEB OF SCIENCETM
Citations

32
checked on Mar 2, 2024

Page view(s)

199
checked on Mar 26, 2024

Download(s)

209
checked on Mar 26, 2024

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.