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https://hdl.handle.net/10316/90918
Title: | Chitosan-Based Coacervate Polymers for Propolis Encapsulation: Release and Cytotoxicity Studies | Authors: | Sato, Tabata Mello, Daphne Vasconcellos, Luana Valente, Artur J. M. Borges, Alexandre Walmott |
Keywords: | DNA; Chitosan; Hydrogel; Pectin; Propolis | Issue Date: | 2020 | Project: | UIDP/QUI/UI0313/2020 UIDB/QUI/UI0313/2020 |
Serial title, monograph or event: | International Journal of Molecular Science | Volume: | 21 | Issue: | 12 | Abstract: | Chitosan-DNA (CS-DNA) and Chitosan-Pectin (CS-P) hydrogels were formulated as a sustained drug delivery carrier for drug delivery. For this, hydrogels were prepared by emulsion technique: mixing aqueous phase of the CS and DNA or P solution with benzyl alcohol using a high-performance dispersing instrument. Green Propolis (GP) was incorporated by imbibition: hydrogels were placed in GP aqueous solution (70 µg/mL) for 2 h. The specimens were freeze-dried and then characterized using different techniques. In vitro cell viability and morphology were also performed using the MG63 cell line. The presence of P was evidenced by the occurrence of a strong band at 1745 cm-1, also occurring in the blend. DNA and CS-DNA showed a strong band at 1650 cm-1, slightly shifted from the chitosan band. The sorption of GP induced a significant modification of the gel surface morphology and some phase separation occurs between chitosan and DNA. Drug release kinetics in water and in saliva follow a two-step mechanism. Significant biocompatibility revealed that these hydrogels were non-toxic and provided acceptable support for cell survival. Thus, the hydrogel complexation of chitosan with DNA and with Pectin provides favorable micro-environment for cell growth and is a viable alternative drug delivery system for Green Propolis. | URI: | https://hdl.handle.net/10316/90918 | ISSN: | 1422-0067 | DOI: | 10.3390/ijms21124561 | Rights: | openAccess |
Appears in Collections: | I&D CQC - Artigos em Revistas Internacionais |
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ijms-21-04561-v2.pdf | 3.61 MB | Adobe PDF | View/Open |
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