Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/8454
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dc.contributor.authorMagalhães-Cardoso, M. Teresa-
dc.contributor.authorPereira, M. Fátima-
dc.contributor.authorOliveira, Laura-
dc.contributor.authorRibeiro, J. A.-
dc.contributor.authorCunha, Rodrigo A.-
dc.contributor.authorCorreia-de-Sá, Paulo-
dc.date.accessioned2009-02-09T14:53:56Z-
dc.date.available2009-02-09T14:53:56Z-
dc.date.issued2003en_US
dc.identifier.citationThe Journal of Physiology. 549:2 (2003) 399-408en_US
dc.identifier.urihttp://hdl.handle.net/10316/8454-
dc.description.abstractAt synapses, ATP is released and metabolised through ecto-nucleotidases forming adenosine, which modulates neurotransmitter release through inhibitory A1 or facilitatory A2A receptors, according to the amounts of extracellular adenosine. Neuromuscular junctions possess an ecto-AMP deaminase that can dissociate extracellular ATP catabolism from adenosine formation. In this study we have investigated the pattern of ATP release and its conversion into adenosine, to probe the role of ecto-AMP deaminase in controlling acetylcholine release from rat phrenic nerve terminals. Nerve-evoked ATP release was 28 ± 12 pmol (mg tissue)22121 at 1 Hz, 54 ± 3 pmol (mg tissue)22121 at 5 Hz and disproportionally higher at 50 Hz (324 ± 23 pmol (mg tissue)22121). Extracellular ATP (30 03BCm) was metabolised with a half time of 8 ± 2 min, being converted into ADP then into AMP. AMP was either dephosphorylated into adenosine by ecto-5'-nucleotidase (inhibited by ATP and blocked by 200 03BCm03B1,03B2-methylene ADP) or deaminated into IMP by ecto-AMP deaminase (inhibited by 200 03BCm deoxycoformycin, which increased adenosine formation). Dephosphorylation and deamination pathways also catabolised endogenously released adenine nucleotides, since the nerve-evoked extracellular AMP accumulation was increased by either 03B1,03B2-methylene ADP (200 03BCm) or deoxycoformycin (200 03BCm). In the presence of nitrobenzylthioinosine (30 03BCm) to inhibit adenosine transport, deoxycoformycin (200 03BCm) facilitated nerve-evoked [3H]acetylcholine release by 77 ± 9 %, an effect prevented by the A2A receptor antagonist, ZM 241385 (10 nm). It is concluded that, while ecto-5'-nucleotidase is inhibited by released ATP, ecto-AMP deaminase activity transiently blunts adenosine formation, which would otherwise reach levels high enough to activate facilitatory A2A receptors on motor nerve terminals.en_US
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleEcto-AMP Deaminase Blunts the ATP-Derived Adenosine A2A Receptor Facilitation of Acetylcholine Release at Rat Motor Nerve Endingsen_US
dc.typearticleen_US
dc.identifier.doi10.1113/jphysiol.2003.040410en_US
item.fulltextCom Texto completo-
item.grantfulltextopen-
item.languageiso639-1en-
Appears in Collections:FMUC Medicina - Artigos em Revistas Internacionais
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