Please use this identifier to cite or link to this item:
Title: Integration of [U-13C]glucose and 2H2O for quantification of hepatic glucose production and gluconeogenesis
Authors: Perdigoto, Rui 
Rodrigues, Tiago B. 
Furtado, Alexandre L. 
Porto, Armando 
Geraldes, Carlos F. G. C. 
Jones, John G. 
Issue Date: 2003
Citation: NMR in Biomedicine. 16:4 (2003) 189-198
Abstract: Glucose metabolism in five healthy subjects fasted for 16 h was measured with a combination of [U-13C]glucose and 2H2O tracers. Phenylbutyric acid was also provided to sample hepatic glutamine for the presence of 13C-isotopomers derived from the incorporation of [U-13C]glucose products into the hepatic Krebs cycle. Glucose production (GP) was quantified by 13C NMR analysis of the monoacetone derivative of plasma glucose following a primed infusion of [U-13C]glucose and provided reasonable estimates (1.90 ± 0.19 mg/kg/min with a range of 1.60-2.15 mg/kg/min). The same derivative yielded measurements of plasma glucose 2H-enrichment from 2H2O by 2H NMR from which the contribution of glycogenolytic and gluconeogenic fluxes to GP was obtained (0.87 ± 0.14 and 1.03 ± 0.10 mg/kg/min, respectively). Hepatic glutamine 13C-isotopomers representing multiply-enriched oxaloacetate and [U-13C]acetyl-CoA were identified as multiplets in the 13C NMR signals of the glutamine moiety of urinary phenylacetylglutamine, demonstrating entry of the [U-13C]glucose tracer into both oxidative and anaplerotic pathways of the hepatic Krebs cycle. These isotopomers contributed 0.1-0.2% excess enrichment to carbons 2 and 3 and sim0.05% to carbon 4 of glutamine. Copyright © 2003 John Wiley & Sons, Ltd.
DOI: 10.1002/nbm.826
Rights: openAccess
Appears in Collections:FMUC Medicina - Artigos em Revistas Internacionais

Files in This Item:
File Description SizeFormat
obra.pdf120.13 kBAdobe PDFView/Open
Show full item record


checked on Feb 18, 2020

Citations 5

checked on Aug 2, 2022

Page view(s) 50

checked on Aug 18, 2022

Download(s) 20

checked on Aug 18, 2022

Google ScholarTM




Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.