Title: Integration of [U-13C]glucose and 2H2O for quantification of hepatic glucose production and gluconeogenesis
Authors: Perdigoto, Rui 
Rodrigues, Tiago B. 
Furtado, Alexandre L. 
Porto, Armando 
Geraldes, Carlos F. G. C. 
Jones, John G. 
Issue Date: 2003
Citation: NMR in Biomedicine. 16:4 (2003) 189-198
Abstract: Glucose metabolism in five healthy subjects fasted for 16 h was measured with a combination of [U-13C]glucose and 2H2O tracers. Phenylbutyric acid was also provided to sample hepatic glutamine for the presence of 13C-isotopomers derived from the incorporation of [U-13C]glucose products into the hepatic Krebs cycle. Glucose production (GP) was quantified by 13C NMR analysis of the monoacetone derivative of plasma glucose following a primed infusion of [U-13C]glucose and provided reasonable estimates (1.90 ± 0.19 mg/kg/min with a range of 1.60-2.15 mg/kg/min). The same derivative yielded measurements of plasma glucose 2H-enrichment from 2H2O by 2H NMR from which the contribution of glycogenolytic and gluconeogenic fluxes to GP was obtained (0.87 ± 0.14 and 1.03 ± 0.10 mg/kg/min, respectively). Hepatic glutamine 13C-isotopomers representing multiply-enriched oxaloacetate and [U-13C]acetyl-CoA were identified as multiplets in the 13C NMR signals of the glutamine moiety of urinary phenylacetylglutamine, demonstrating entry of the [U-13C]glucose tracer into both oxidative and anaplerotic pathways of the hepatic Krebs cycle. These isotopomers contributed 0.1-0.2% excess enrichment to carbons 2 and 3 and sim0.05% to carbon 4 of glutamine. Copyright © 2003 John Wiley & Sons, Ltd.
URI: http://hdl.handle.net/10316/8430
Rights: openAccess
Appears in Collections:FMUC Medicina - Artigos em Revistas Internacionais

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