Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/8336
Title: Cardosins: A new and efficient plant enzymatic tool to dissociate neuronal cells for the establishment of cell cultures
Authors: Duarte, A. S. 
Rosa, N. 
Duarte, E. P. 
Pires, E. 
Barros, M. T. 
Issue Date: 2007
Citation: Biotechnology and Bioengineering. 97:4 (2007) 991-996
Abstract: In the present work, we examined the feasibility of using cardosins, plant aspartic-proteinases from Cynara cardunculus L., to isolate cells from rat embryonic brain. Using morphological and functional assays, we compared cell cultures obtained with cardosins with those prepared with a well-established trypsin protocol. Cardosins and trypsin dissociation produced cells with similar yield, viability, and GABA release in response to a depolarizing stimulus. However, cardosins-dissociated cells appeared to recover faster in culture, as assessed by the MTT-test and by the number and length of neurtites, suggesting that cardosins are less aggressive to neurons than trypsin. This feature might be helpful for research and medical purposes requiring fast manipulations of cells. Biotechnol. Bioeng. 2007;97: 991-996. © 2006 Wiley Periodicals, Inc.
URI: http://hdl.handle.net/10316/8336
DOI: 10.1002/bit.21259
Rights: openAccess
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais

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