Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/8329
DC Field | Value | Language |
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dc.contributor.author | Caramelo, Olga L. | - |
dc.contributor.author | Santos, Paulo F. | - |
dc.contributor.author | Carvalho, Arsélio P. | - |
dc.contributor.author | Duarte, Carlos B. | - |
dc.date.accessioned | 2009-02-09T14:33:41Z | - |
dc.date.available | 2009-02-09T14:33:41Z | - |
dc.date.issued | 1999 | en_US |
dc.identifier.citation | Journal of Neuroscience Research. 58:4 (1999) 505-514 | en_US |
dc.identifier.uri | https://hdl.handle.net/10316/8329 | - |
dc.description.abstract | Retinal amacrine cells express metabotropic glutamate receptors (mGluRs), but their physiological role is unknown. We investigated the effect of mGluR on [3H]acetylcholine release ([3H]ACh) from cultured chick amacrine-like neurons. Activation of group III mGluR with the agonist L(+)-2-amino-4-phosphonobutyric acid (L-AP4) inhibited [3H]ACh release evoked by 25 mM KCl in a dose-dependent manner, and this effect was sensitive to pertussis toxin. In contrast, activation of group I or II mGluR with (S)-3,5-dihydroxyphenylglycine (DHPG) and (2S,2primeR,3primeR)-2-(2prime,3prime-dicarboxycyclopropyl)glycine (DCG-IV), respectively, did not affect significantly [3H]ACh release. The effect of L-AP4 on [3H]ACh release was sensitive to nitrendipine, suggesting that it is, at least in part, due to inhibition of L-type Ca2+ channels. Activation of group III mGluR also partly inhibited ω-conotoxin GVIA-sensitive Ca2+ channels, coupled to [3H]ACh release. The L-AP4 did not affect the cAMP levels measured in amacrine-like neurons depolarized with 25 mM KCl or stimulated with forskolin, indicating that the effect of group III mGluR on [3H]ACh release is not due to inhibition of adenylyl cyclase activity. Inhibition of protein kinase A with KT-5720 was without effect on [3H]ACh release evoked by 25 mM KCl, further indicating that the effect of group III mGluR on [3H]ACh release cannot be attributed to the inhibition of the kinase. The effect of L-AP4 on [3H]ACh release was reversed by DHPG or by DCG-IV, and activation of group II mGluR also partially inhibited cAMP production stimulated by forskolin. Taken together, our results show that the effect of group III mGluR on [3H]ACh release may be due to a direct inhibition of L- and N-type Ca2+ channels and is modulated by group I and group II mGluR. J. Neurosci. Res. 58:505-514, 1999. © 1999 Wiley-Liss, Inc. | en_US |
dc.language.iso | eng | eng |
dc.rights | openAccess | eng |
dc.title | Metabotropic glutamate receptors modulate [3H]acetylcholine release from cultured amacrine-like neurons | en_US |
dc.type | article | en_US |
dc.identifier.doi | 10.1002/(SICI)1097-4547(19991115)58:4<505::AID-JNR4>3.0.CO;2-J | en_US |
uc.controloAutoridade | Sim | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.openairetype | article | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.fulltext | Com Texto completo | - |
item.languageiso639-1 | en | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0002-2225-455X | - |
crisitem.author.orcid | 0000-0002-1474-0208 | - |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
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