Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/8238
DC FieldValueLanguage
dc.contributor.authorMendes, Ana C.-
dc.contributor.authorCaldeira, M. Madalena-
dc.contributor.authorSilva, Cláudia-
dc.contributor.authorBurgess, Shawn C.-
dc.contributor.authorMerritt, Matthew E.-
dc.contributor.authorGomes, Filipe-
dc.contributor.authorBarosa, Cristina-
dc.contributor.authorDelgado, Teresa C.-
dc.contributor.authorFranco, Fátima-
dc.contributor.authorMonteiro, Pedro-
dc.contributor.authorProvidência, Luís-
dc.contributor.authorJones, John G.-
dc.date.accessioned2009-02-09T14:32:10Z-
dc.date.available2009-02-09T14:32:10Z-
dc.date.issued2006en_US
dc.identifier.citationMagnetic Resonance in Medicine. 56:5 (2006) 1121-1125en_US
dc.identifier.urihttp://hdl.handle.net/10316/8238-
dc.description.abstractMenthol glucuronide was isolated from the urine of a healthy 70-kg female subject following ingestion of 400 mg of peppermint oil and 6 g of 99% [U-13C]glucose. Glucuronide 13C-excess enrichment levels were 4-6% and thus provided high signal-to-noise ratios (SNRs) for confident assignment of 13C-13C spin-coupled multiplet components within each 13C resonance by 13C NMR. The [U-13C]glucuronide isotopomer derived via direct pathway conversion of [U-13C]glucose to [U-13C]UDP-glucose was resolved from [1,2,3-13C3]- and [1,2-13C2]glucuronide isotopomers derived via Cori cycle or indirect pathway metabolism of [U-13C]glucose. In a second study, a group of four overnight-fasted patients (63 ± 10 kg) with severe heart failure were given peppermint oil and infused with [U-13C]glucose for 4 hr (14 mg/kg prime, 0.12 mg/kg/min constant infusion) resulting in a steady-state plasma [U-13C]glucose enrichment of 4.6% ± 0.6%. Menthol glucuronide was harvested and glucuronide 13C-isotopomers were analyzed by 13C NMR. [U-13C]glucuronide enrichment was 0.6% ± 0.1%, and the sum of [1,2,3-13C3] and [1,2-13C2]glucuronide enrichments was 0.9% ± 0.2%. From these data, flux of plasma glucose to hepatic UDPG was estimated to be 15% ± 4% that of endogenous glucose production (EGP), and the Cori cycle accounted for at least 32% ± 10% of GP. Magn Reson Med, 2006. © 2006 Wiley-Liss, Inc.en_US
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleHepatic UDP-glucose 13C isotopomers from [U-13C]glucose: A simple analysis by 13C NMR of urinary menthol glucuronideen_US
dc.typearticleen_US
dc.identifier.doi10.1002/mrm.21057en_US
item.grantfulltextopen-
item.fulltextCom Texto completo-
item.languageiso639-1en-
crisitem.author.deptCNC - Center for Neuroscience and Cell Biology-
crisitem.author.orcid0000-0002-3745-3885-
Appears in Collections:FCTUC Química - Artigos em Revistas Internacionais
Files in This Item:
File Description SizeFormat
obra.pdf106.93 kBAdobe PDFView/Open
Show simple item record

SCOPUSTM   
Citations

13
checked on Feb 18, 2020

WEB OF SCIENCETM
Citations 5

16
checked on Aug 2, 2022

Page view(s)

263
checked on Sep 15, 2022

Download(s)

176
checked on Sep 15, 2022

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.